Tsunekawa Taichi, Kaneko Hiroki, Takayama Kei, Hwang Shiang-Jyi, Oishi Akio, Nagasaka Yosuke, Ye Fuxiang, Iwase Takeshi, Nonobe Norie, Ueno Shinji, Ito Yasuki, Yasuda Shunsuke, Matsuura Toshiyuki, Shimizu Hideyuki, Suzumura Ayana, Kataoka Keiko, Terasaki Hiroko
Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Laboratory of Bell Research Center-Department of Obstetrics and Gynecology Collaborative Research, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Biomed Res Int. 2017;2017:3427319. doi: 10.1155/2017/3427319. Epub 2017 Feb 5.
We had earlier reported positive hsa-miR-148a-3p expression in eyes with rhegmatogenous retinal detachment (RRD) and its involvement in the epithelial-mesenchymal transition of retinal pigment epithelium in vitro. Here we investigated the association of hsa-miR-148a-3p expression levels in the vitreous fluid of patients with RRD with severity of RRD. The hsa-miR-148a-3p expression levels in the vitreous fluid, range (degree) of retinal detachment (RD), and pixels of retinal break were measured in 27 eyes with RRD. The association of hsa-miR-148a-3p expression levels with other factors was evaluated by multiple regression analysis. The hsa-miR-148a-3p expression levels, time from onset of RRD to vitrectomy, range of RD, and pixels of retinal breaks were 23.68 ± 43.00, 12.07 ± 15.36 days, 155.85 ± 86.67 degrees, and 37000 ± 67100 pixels, respectively. Five eyes with RRD had vitreous hemorrhage preoperatively. The hsa-miR-148a-3p expression levels were significantly associated with pixels of retinal breaks ( = 0.699) and the time from onset of RRD to vitrectomy ( = 0.358) but not with the range of RD or presence of vitreous hemorrhage. The hsa-miR-148a-3p expression levels in the vitreous fluid were significantly associated with the size of retinal break and disease duration.
我们之前报道过，孔源性视网膜脱离（RRD）患者眼中hsa-miR-148a-3p呈阳性表达，且其在体外参与视网膜色素上皮的上皮-间质转化。在此，我们研究了RRD患者玻璃体液中hsa-miR-148a-3p表达水平与RRD严重程度之间的关联。我们检测了27只RRD患眼玻璃体液中hsa-miR-148a-3p的表达水平、视网膜脱离（RD）的范围（程度）以及视网膜裂孔的像素数。通过多元回归分析评估hsa-miR-148a-3p表达水平与其他因素的关联。玻璃体液中hsa-miR-148a-3p的表达水平、RRD发病至玻璃体切除的时间、RD范围以及视网膜裂孔的像素数分别为23.68±43.00、12.07±15.36天、155.85±86.67度和37000±67100像素。5只RRD患眼术前存在玻璃体积血。hsa-miR-148a-3p表达水平与视网膜裂孔的像素数（r = 0.699）以及RRD发病至玻璃体切除的时间（r = 0.358）显著相关，但与RD范围或玻璃体积血的存在无关。玻璃体液中hsa-miR-148a-3p的表达水平与视网膜裂孔大小和病程显著相关。
