雌激素诱导禽类肝脏卵黄蛋白原生物合成过程中功能性白蛋白mRNA的减少。
Decrease in functional albumin mRNA during estrogen-induced vitellogenin biosynthesis in avian liver.
作者信息
Williams D L, Wang S Y, Klett H
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5974-8. doi: 10.1073/pnas.75.12.5974.
Translation of rooster liver RNA in a wheat germ extract is shown to yield albumin as one of the cell-free products. Quantitation of albumin mRNA by the translation assay indicates that functional albumin mRNA represents 10% of total liver mRNA activity in control roosters. After estrogen administration, this level decreases in a continuous fashion until functional albumin mRNA represents 5% of total mRNA activity at 12 days. This decrease in functional albumin mRNA was correlated with several parameters of vitellogenin induction. Functional vitellogenin mRNA increases to a maximum at 4 days after hormone treatment and returns to control levels by 12 days. A similar pattern is seen for the hormone-stimulated increase in total mRNA activity. The decrease in functional albumin mRNA, therefore, persists after the vitellogenic response of the liver has been completed. These results suggest that the decrease in hepatic albumin synthesis after hormone treatment is due to an estrogen-mediated decrease in the content of albumin mRNA.
在小麦胚芽提取物中对公鸡肝脏RNA进行翻译，结果显示无细胞产物之一是白蛋白。通过翻译测定法对白蛋白mRNA进行定量分析表明，在对照公鸡中，功能性白蛋白mRNA占肝脏总mRNA活性的10%。给予雌激素后，这一水平持续下降，直至12天时功能性白蛋白mRNA占总mRNA活性的5%。功能性白蛋白mRNA的这种下降与卵黄蛋白原诱导的几个参数相关。功能性卵黄蛋白原mRNA在激素处理后第4天增加到最大值，并在12天时恢复到对照水平。激素刺激引起的总mRNA活性增加也呈现类似模式。因此，在肝脏的卵黄生成反应完成后，功能性白蛋白mRNA的下降仍然存在。这些结果表明，激素处理后肝脏白蛋白合成的减少是由于雌激素介导的白蛋白mRNA含量下降所致。
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