Long Hua-Li, Zhang Jing-Xian, Guo Ji-Ling, Sui Guo-Qi, Hou Jin-Jun, Yang Wen-Zhi, Han Su-Mei, Wu Wan-Ying, Guo De-An
National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Chin J Nat Med. 2016 Dec;14(12):954-960. doi: 10.1016/S1875-5364(17)30022-5.
Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers. However, the assessment criteria always show a poor specificity, due to the lack of systematic comparison among the analogous herbs. In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control. Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed. Based upon the quantitative results, the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7% were proposed for sea-tangle and sargassum, respectively. Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs. Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components. A discriminative criterion using the peak area ratio of these two monosaccharides derivatives was proposed for the qualitative identification. In conclusion, a more specific and quantitative quality control method was developed for sea-tangle and sargassum.
众多传统中药中的多糖已被证明是生物活性成分，因此被用作质量控制指标。然而，由于缺乏对类似药材的系统比较，评估标准的特异性往往较差。在本研究中，选择两种相似的材料，即海带和马尾藻，作为模型药材，以开发更具特异性的质量控制方法。采用了两种成熟的方法，即总多糖含量测定和单糖组成分析。基于定量结果，分别提出了海带和马尾藻多糖含量不低于2.0%和1.7%的评价标准。两种药材水解和衍生化溶液的高效液相色谱图上出现了9种相同的单糖衍生物。以单糖衍生物的峰面积为变量进行主成分分析和正交偏最小二乘判别分析，结果表明两种药材中浓度相反的甘露糖醛酸和木糖是差异成分。提出了以这两种单糖衍生物的峰面积比作为定性鉴别的判别标准。总之，建立了一种更具特异性和定量性的海带和马尾藻质量控制方法。
