Gao Jie, Lin Lianzhu, Sun Baoguo, Zhao Mouming
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Food Funct. 2017 Sep 20;8(9):3043-3052. doi: 10.1039/c7fo00218a.
In this study, the structural characterization and bile acid-binding capacity of Laminaria japonica polysaccharides (LP), obtained by seven different extraction methods, were investigated. The results indicated that extraction methods exhibited significant effects on extraction yield, molecular weight, monosaccharide composition and the content of neutral sugar, fucose, uronic acid and sulfate of LP. AFM analysis indicated that LP extracted by different methods exhibited certain different, flexible and worm-like chains with many branches. Rheological measurements showed that the LP, obtained by pressurized hot water extraction and acid assisted extraction, exhibited lower viscosity due to their lower molecular weight, compared to other extracted polysaccharides. The bile acid-binding capacity of acid assisted extracted LP was significantly higher than other LP samples tested, which was probably ascribed to its highly branched structure, low molecular weight, low viscosity and abundant uronic acid and fucose in total monosaccharides. The present study provides scientific evidence and advances in the preparation technology and a method for evaluating hypolipidemic activities of L. japonica polysaccharides.
本研究对通过七种不同提取方法获得的海带多糖（LP）的结构特征和胆汁酸结合能力进行了研究。结果表明，提取方法对LP的提取率、分子量、单糖组成以及中性糖、岩藻糖、糖醛酸和硫酸盐的含量有显著影响。原子力显微镜分析表明，不同方法提取的LP呈现出一定的差异，具有许多分支的柔性蠕虫状链。流变学测量表明，与其他提取的多糖相比，通过加压热水提取和酸辅助提取获得的LP由于分子量较低而表现出较低的粘度。酸辅助提取的LP的胆汁酸结合能力明显高于其他测试的LP样品，这可能归因于其高度分支的结构、低分子量、低粘度以及总单糖中丰富的糖醛酸和岩藻糖。本研究为海带多糖的制备技术和降血脂活性评价方法提供了科学依据和进展。
