来自土壤杆菌属菌株ATCC 21400的纤维二糖酶编码基因的结构与转录分析
Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400.
作者信息
Wakarchuk W W, Greenberg N M, Kilburn D G, Miller R C, Warren R A
机构信息
Department of Microbiology, University of British Columbia, Vancouver, Canada.
出版信息
J Bacteriol. 1988 Jan;170(1):301-7. doi: 10.1128/jb.170.1.301-307.1988.
The DNA sequence was determined for the cloned Agrobacterium sp. strain ATCC 21400 beta-glucosidase gene, abg. High-resolution nuclease S1 protection studies were used to map the abg mRNA 5' and 3' termini. A putative abg promoter was identified whose sequence shows similarities to the consensus promoter of Escherichia coli and with the nif promoter regions of Klebsiella. The abg coding sequence was 1,374 nucleotides long. The molecular weight of the enzyme, based on the predicted amino acid sequence, was 51,000. The observed Mr was 50,000 to 52,000. A region of deduced protein sequence was homologous to a region from two other beta-glucosidase sequences. This region of homology contained a putative active site by analogy with the active site of hen egg white lysozyme.
测定了克隆的土壤杆菌属菌株ATCC 21400的β-葡萄糖苷酶基因（abg）的DNA序列。采用高分辨率核酸酶S1保护研究来绘制abg mRNA的5'和3'末端图谱。鉴定出一个假定的abg启动子，其序列与大肠杆菌的共有启动子以及克雷伯氏菌的nif启动子区域相似。abg编码序列长1374个核苷酸。根据预测的氨基酸序列，该酶的分子量为51,000。观察到的Mr为50,000至52,000。推导的蛋白质序列区域与另外两个β-葡萄糖苷酶序列的一个区域同源。通过与鸡蛋清溶菌酶的活性位点类比，该同源区域包含一个假定的活性位点。
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