Braz G R F, Emiliano A S, Sousa S M, Pedroza A A S, Santana D F, Fernandes M P, da Silva A I, Lagranha C J
1Laboratory of Biochemistry and Exercise Biochemistry,CAV-Federal University of Pernambuco,Pernambuco,Brazil.
J Dev Orig Health Dis. 2017 Jun;8(3):322-330. doi: 10.1017/S2040174417000058. Epub 2017 Mar 7.
Several studies have shown that maternal low-protein (LP) diet induces detrimental effects in cardiovascular system and oxidative stress in male animals. Additional studies suggested that female has lower incidence of cardiovascular disease. However until present data, the possible effects of estradiol on the undernutrition during gestational and lactation periods are not discussed. The present study was conducted to evaluate the effects of a maternal LP diet during gestational and lactation period on oxidative balance in the female rat hearts ventricles at two ages. Dams were fed with normal protein (NP) or a LP diet during the gestational and lactation period, and their female offspring were divided into age groups (22 or 122 days, corresponding to a low or high estrogen level) composing four experimental groups. Evaluating the nutritional effect showed an increase in oxidative stress biomarkers and decrease in enzymatic defense in LP-22D compared with NP-22D. In contrast, no changes were observed in malondialdehyde and carbonyls, but an increase in glutathione-S-transferase (GST) activity in the LP-122D compared with NP-122D. The global oxy-score in the LP-22D group indicated a predominance of oxidative damage when compared with NP-22D, while in LP-122D group the global oxy-score was restored to NP-122D levels. Evaluating the estradiol effect, our data show a significant decrease in oxidative stress with increase in CAT and GST activity, associated with increase in intracellular thiols. Our data suggest that in situation with low levels of estradiol, hypoproteic diet during gestation and lactation period has detrimental effects on heart, however when estradiol levels raise, the detrimental effects induced are mitigated.
多项研究表明，母体低蛋白（LP）饮食会对雄性动物的心血管系统和氧化应激产生有害影响。另外的研究表明，女性患心血管疾病的几率较低。然而，直至目前的数据，尚未讨论雌二醇在妊娠期和哺乳期对营养不良可能产生的影响。本研究旨在评估妊娠期和哺乳期母体LP饮食对两个年龄段雌性大鼠心室氧化平衡的影响。在妊娠期和哺乳期，给母鼠喂食正常蛋白（NP）或LP饮食，其雌性后代被分为年龄组（22天或122天，分别对应低雌激素水平或高雌激素水平），组成四个实验组。评估营养效应显示，与NP - 22D组相比，LP - 22D组氧化应激生物标志物增加，酶防御能力下降。相比之下，丙二醛和羰基没有变化，但与NP - 122D组相比，LP - 122D组谷胱甘肽 - S - 转移酶（GST）活性增加。与NP - 22D组相比，LP - 22D组的整体氧化评分表明氧化损伤占主导，而在LP - 122D组中，整体氧化评分恢复到NP - 122D组的水平。评估雌二醇的作用，我们的数据显示氧化应激显著降低，同时CAT和GST活性增加，细胞内硫醇也增加。我们的数据表明，在雌二醇水平较低的情况下，妊娠期和哺乳期的低蛋白饮食对心脏有有害影响，然而当雌二醇水平升高时，所诱导的有害影响会减轻。
