Pinget M, Straus E, Yalow R S
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6324-6. doi: 10.1073/pnas.75.12.6324.
Subcellular fractionation of the rat cerebral cortex demonstrated the presence of immunoreactive cholecystokinin in the pellet identified by electron microscopy as containing a high proportion of synaptic vesicles. The recovery in this pellet of 40% of the total immunoreactivity in the initial cortical extract is quite comparable to the recovery of other peptides such as vasoactive intestinal polypeptide and somatostatin, which are also located in synaptosomes and for which roles as neuroregulators or transmitters have been suggested. The evidence of concentration of cholecystokinin-like peptides in the synaptosomal pellet is consistent with our earlier demonstration by immunohistochemical techniques of cholecystokinin's presence in rabbit cerebral cortical neurons. These observations and the evidence for diminished concentration of cholecystokinin-like peptides in the brains of hyperphagic mice are consistent with cholecystolinin's suggested role as a neuroregulator for appetite.
对大鼠大脑皮层进行亚细胞分级分离显示，在经电子显微镜鉴定含有高比例突触小泡的沉淀中存在免疫反应性胆囊收缩素。该沉淀中回收了初始皮层提取物中40%的总免疫反应性，这与其他肽如血管活性肠肽和生长抑素的回收率相当，这些肽也位于突触体中，并且有人提出它们具有神经调节因子或神经递质的作用。突触体沉淀中胆囊收缩素样肽的浓缩证据与我们早期通过免疫组织化学技术证明胆囊收缩素存在于兔大脑皮层神经元中的结果一致。这些观察结果以及贪食小鼠大脑中胆囊收缩素样肽浓度降低的证据与胆囊收缩素作为食欲神经调节因子的推测作用是一致的。
