Morino P, Herrera-Marschitz M, Castel M N, Ungerstedt U, Varro A, Dockray G, Hökfelt T
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Eur J Neurosci. 1994 May 1;6(5):681-92. doi: 10.1111/j.1460-9568.1994.tb00980.x.
Using immunohistochemical techniques we have analysed the occurrence of cholecystokinin-like immunoreactivity (CCK-LI) in the cortex and striatum of the rat. In the cortex few CCK-immunoreactive cell bodies, mainly interneurons, could be visualized in normal brains, and a moderately dense network of CCK fibres was also observed. Injections of colchicine into the striatum led to an accumulation, in the surrounding cortex, of CCK-LI in the initial segment of the axon of numerous cells. In addition, with an antibody to pro-CCK several cell bodies, many of which with pyramidal shape, could be visualized. Furthermore, retrograde staining of cortical cells after unilateral injection of wheat germ agglutinin into the striatum revealed bilaterally in the cortex a number of labelled cells that also contained pro-CCK-LI. In the striatum CCK-LI was diffusely distributed in fine fibres as well as in patches of fibres located in the medial aspects. After decortication followed by callosotomy these patches disappeared on the side ipsilateral to the lesion, while the pattern of immunoreactivity of several other peptides in the striatum was unaffected. No change was observed on the contralateral side. Decortication or callosotomy alone did not affect the pattern of CCK-LI. At the ultrastructural level several CCK-immunoreactive terminals could be observed, mostly with clear, densely packed vesicles and straight asymmetric synaptic contacts with small spines, characteristic for terminals of cortical origin. The results are consistent with the presence of a major, partly crossed, CCK-containing cortico-striatal pathway.
我们运用免疫组织化学技术分析了大鼠大脑皮质和纹状体中胆囊收缩素样免疫反应性（CCK-LI）的出现情况。在正常大脑的皮质中，少数CCK免疫反应性细胞体（主要是中间神经元）清晰可见，同时还观察到了中等密度的CCK纤维网络。向纹状体内注射秋水仙碱后，在周围皮质中，许多细胞轴突的起始段出现了CCK-LI的积聚。此外，使用抗前CCK抗体可使多个细胞体显现出来，其中许多呈锥体形。而且，在向纹状体内单侧注射小麦胚凝集素后对皮质细胞进行逆行染色，结果显示双侧皮质中有许多标记细胞也含有前CCK-LI。在纹状体内，CCK-LI弥散分布于细纤维以及位于内侧的纤维斑中。在进行去皮质术并切断胼胝体后，这些纤维斑在损伤同侧消失，而纹状体内其他几种肽的免疫反应模式未受影响。对侧未见变化。单独进行去皮质术或切断胼胝体均未影响CCK-LI的模式。在超微结构水平上，可以观察到几个CCK免疫反应性终末，大多数终末含有清亮、密集堆积的囊泡，并与小棘形成直的不对称突触联系，这是皮质起源终末的特征。这些结果与存在一条主要的、部分交叉的含CCK的皮质 - 纹状体通路相一致。
