Ahmad F, Young H, McLeod D T, Croughan M J, Calder M A
Department of Bacteriology, City Hospital, Edinburgh, Scotland.
J Clin Pathol. 1987 Nov;40(11):1369-73. doi: 10.1136/jcp.40.11.1369.
To distinguish Branhamella catarrhalis from Neisseria species a study of 140 strains was made on simple laboratory media, with particular reference to deoxyribonuclease (DNase) production, superoxol reaction, and growth characteristics. All 97 clinical isolates of B catarrhalis (58 of which were beta-lactamase positive) and eight strains of B catarrhalis from the National Collection of Type Cultures were DNase positive and superoxol positive. None grew on modified New York City medium, modified Thayer Martin medium, MacConkey agar, crystal violet blood agar, nor under anaerobic conditions. Of the 16 different non-pathogenic Neisseria species tested, all were DNase negative, eight (50%) were superoxol reaction negative, and 13 (81%) grew on crystal violet blood agar. Using simple laboratory media, DNase, and superoxol tests, it was possible to identify B catarrhalis and to distingish it from pathogenic and non-pathogenic Neisseria species.
为了将卡他布兰汉菌与奈瑟菌属区分开来，我们在简单的实验室培养基上对140株菌株进行了研究，特别参考了脱氧核糖核酸酶（DNase）的产生、过氧乙酸反应及生长特性。所有97株卡他布兰汉菌临床分离株（其中58株β-内酰胺酶阳性）以及来自国家典型培养物保藏中心的8株卡他布兰汉菌均为DNase阳性和过氧乙酸阳性。在改良纽约市培养基、改良泰-马培养基、麦康凯琼脂、结晶紫血琼脂上均不生长，在厌氧条件下也不生长。在测试的16种不同的非致病性奈瑟菌属中，所有菌株均为DNase阴性，8株（50%）过氧乙酸反应阴性，13株（81%）在结晶紫血琼脂上生长。利用简单的实验室培养基、DNase和过氧乙酸试验，可以鉴定卡他布兰汉菌，并将其与致病性和非致病性奈瑟菌属区分开来。
