Janda W M, Zigler K L, Bradna J J
J Clin Microbiol. 1987 Feb;25(2):203-6. doi: 10.1128/jcm.25.2.203-206.1987.
The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The two misidentified meningococcal strains were biochemically atypical and were also misidentified by the conventional method. Of 26 N. lactamica strains, 25 (96%) were correctly identified. Of 21 Neisseria spp., 14 (67%) produced carbohydrate reactions in agreement with the conventional procedure, and 7 strains produced detectable acid in the QuadFERM+ from maltose and sucrose but not glucose. All 9 N. cinerea and 30 B. catarrhalis strains were asaccharolytic by QuadFERM+. The rapid DNase test was positive for all B. catarrhalis strains and negative for all other organisms. Two beta-lactamase-positive N. gonorrhoeae strains and 25 (93%) of 27 beta-lactamase-positive B. catarrhalis strains were detected by the 2-h acidometric beta-lactamase test on the strip. QuadFERM+ with rapid DNase is a simple and easily interpretable method for identification of these organisms in the clinical laboratory.
QuadFERM+系统（Analytab Products公司，纽约州普莱恩维尤）是一种用于鉴定奈瑟菌属的2小时碳水化合物降解方法，我们对其进行了评估，并采用快速DNase试验来确认卡他布兰汉菌。QuadFERM+系统鉴定出82株淋病奈瑟菌中的100%以及54株脑膜炎奈瑟菌中的96%。两株被误鉴定的脑膜炎奈瑟菌菌株在生化特性上不典型，采用传统方法也出现了误鉴定。在26株乳糖奈瑟菌菌株中，25株（96%）被正确鉴定。在21株奈瑟菌属菌株中，14株（67%）产生的碳水化合物反应与传统方法一致，7株菌株在QuadFERM+系统中从麦芽糖和蔗糖而非葡萄糖中产生了可检测到的酸。所有9株灰色奈瑟菌和30株卡他布兰汉菌菌株在QuadFERM+系统中均不分解糖类。快速DNase试验对所有卡他布兰汉菌菌株呈阳性，对所有其他菌株呈阴性。通过纸条上的2小时酸度法β-内酰胺酶试验检测到2株β-内酰胺酶阳性的淋病奈瑟菌菌株以及27株β-内酰胺酶阳性的卡他布兰汉菌菌株中的25株（93%）。QuadFERM+系统结合快速DNase试验是临床实验室鉴定这些微生物的一种简单且易于解读的方法。
