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通过标准和实验测试鉴定问题淋病奈瑟菌培养物。

Identification of problem Neisseria gonorrhoeae cultures by standard and experimental tests.

作者信息

Arko R J, Finley-Price K G, Wong K H, Johnson S R, Reising G

出版信息

J Clin Microbiol. 1982 Mar;15(3):435-8. doi: 10.1128/jcm.15.3.435-438.1982.

Abstract

Standard and experimental tests were used by a reference diagnostic laboratory to determine the identity of 182 "suspected" Neisseria gonorrhoeae isolates submitted by state health departments because of inconclusive laboratory results. More than 97% of these cultures were subsequently identified by a rapid microcarbohydrate test in conjunction with confirmatory immunological procedures. The experimental rapid slide agglutination test using rough-lipopolysaccharide antibody, the Phadebact co-agglutination test, and fluorescent antibody test identified 49.3 to 94.1% of these cultures. Because of frequent problems with carbohydrate utilization, Neisseria meningitidis and Branhamella catarrhalis were the two microorganisms most often confused with N. gonorrhoeae by submitting laboratories.

摘要

一家参考诊断实验室使用标准和实验测试来确定各州卫生部门提交的182株“疑似”淋病奈瑟菌分离株的身份,这些分离株由于实验室结果不确定而被提交。随后,超过97%的这些培养物通过快速微量碳水化合物测试结合确证性免疫程序得以鉴定。使用粗糙脂多糖抗体的实验性快速玻片凝集试验、协同凝集试验和荧光抗体试验鉴定出了这些培养物中的49.3%至94.1%。由于碳水化合物利用方面经常出现问题,脑膜炎奈瑟菌和卡他布兰汉菌是提交实验室最常与淋病奈瑟菌混淆的两种微生物。

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