Laboratory of Growth Regulators, Faculty of Science, Institute of Experimental Botany of the Czech Academy of Sciences, Palacký University, Šlechtitelů 27, CZ-78371 Olomouc, Czech Republic.
Department of Biophysics, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-78371 Olomouc, Czech Republic.
Biomolecules. 2023 Feb 5;13(2):299. doi: 10.3390/biom13020299.
is an obligate biotrophic pathogen causing clubroot disease in cruciferous plants. Infected plant organs are subject to profound morphological changes, the roots form characteristic galls, and the leaves are chlorotic and abscise. The process of gall formation is governed by timely changes in the levels of endogenous plant hormones that occur throughout the entire life cycle of the clubroot pathogen. The homeostasis of two plant hormones, cytokinin and auxin, appears to be crucial for club development. To investigate the role of cytokinin and auxin in gall formation, we used metabolomic and transcriptomic profiling of infected with clubroot, focusing on the late stages of the disease, where symptoms were more pronounced. Loss-of-function mutants of three cytokinin receptors, AHK2, AHK3, and CRE1/AHK4, were employed to further study the homeostasis of cytokinin in response to disease progression; double mutants developed characteristic symptoms of the disease, albeit with varying intensity. The most susceptible to clubroot disease was the double mutant, as revealed by measuring its photosynthetic performance. Quantification of phytohormone levels and pharmacological treatment with the cytokinin antagonist PI-55 showed significant changes in the levels of endogenous cytokinin and auxin, which was manifested by both enhanced and reduced development of disease symptoms in different genotypes.
是一种专性生物营养型病原体，可引起十字花科植物的根肿病。受感染的植物器官会发生明显的形态变化，根部形成特征性的瘿瘤，叶片出现黄化和脱落。瘿瘤的形成过程受整个根肿病生命周期中内源性植物激素水平的适时变化所调控。细胞分裂素和生长素这两种植物激素的动态平衡似乎对根肿病的发展至关重要。为了研究细胞分裂素和生长素在瘿瘤形成中的作用，我们利用代谢组学和转录组学对感染的组织进行了分析，重点关注疾病的后期阶段，此时症状更为明显。我们使用三个细胞分裂素受体（AHK2、AHK3 和 CRE1/AHK4）的功能丧失突变体，进一步研究了细胞分裂素在应对疾病进展中的动态平衡；双突变体表现出了该疾病的典型症状，尽管强度有所不同。通过测量其光合性能，发现 双突变体对根肿病最为敏感。对植物激素水平的定量分析以及用细胞分裂素拮抗剂 PI-55 进行的药理学处理表明，内源细胞分裂素和生长素水平发生了显著变化，这在不同基因型中均表现为疾病症状的增强和减轻。
