Li Haixia, Shi Baoyuan, Li Yanyun, Yin Fengfang
Department of Otolaryngology, Huaihe Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
Department of Obstetrics and Gynecology, Huaihe Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
J Biochem Mol Toxicol. 2017 Jul;31(7). doi: 10.1002/jbt.21900. Epub 2017 Mar 7.
Polydatin (PD), a stilbene compound extracted from Polygonum cuspidatum, is suggested to possess anti-cancer activities, including inhibition of cell proliferation, cell cycle arrest, and induction of apoptosis. The platelet-derived growth factor (PDGF)/AKT signaling pathway plays complex roles in tumor suppression. However, the effect of PD on the PDGF/AKT signaling pathway in laryngeal cancer and HeLa cells has not been explored. MTT assay and flow cytometry showed that PD inhibited cell proliferation and induced apoptosis in Hep-2 and AMC-HN-8 cells. Western blot analysis indicated that PD inhibited the expression levels of PDGF-B and phosphorylated AKT (p-AKT) in both cells. Treatment of PDGF-B siRNA or PDGFR inhibitor found that after the PDGF signaling was inactivated, p-AKT expression was significantly decreased in Hep-2 cells. Tumor xenograft experiment in nude mice indicated PD significantly inhibited the growth of Hep-2 cells in vivo. In conclusion, PD inhibited cell proliferation and induced apoptosis in laryngeal cancer and HeLa cells via inactivation of the PDGF/AKT signaling pathway.
虎杖苷（PD）是一种从虎杖中提取的芪类化合物，具有抗癌活性，包括抑制细胞增殖、使细胞周期停滞以及诱导细胞凋亡。血小板衍生生长因子（PDGF）/AKT信号通路在肿瘤抑制中发挥着复杂的作用。然而，PD对喉癌和HeLa细胞中PDGF/AKT信号通路的影响尚未得到研究。MTT法和流式细胞术表明，PD抑制Hep-2和AMC-HN-8细胞的增殖并诱导其凋亡。蛋白质印迹分析表明，PD抑制这两种细胞中PDGF-B和磷酸化AKT（p-AKT）的表达水平。用PDGF-B小干扰RNA（siRNA）或PDGFR抑制剂处理发现，在PDGF信号失活后，Hep-2细胞中p-AKT的表达显著降低。裸鼠肿瘤异种移植实验表明，PD在体内显著抑制Hep-2细胞的生长。总之，PD通过使PDGF/AKT信号通路失活来抑制喉癌和HeLa细胞的增殖并诱导其凋亡。
