Pan Ji-Hong, Wang Hai-Bin, Du Xiao-Fei, Liu Jiang-Yue, Zhang Dai-Juan
Department of Oncology, Rizhao Hospital of Traditional Chinese Medicine, Rizhao 276800, China.
People's Hospital of Rizhao, Rizhao 276800, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jun;42(12):2345-2349. doi: 10.19540/j.cnki.cjcmm.2017.0111.
To observe the effect of polydatin on proliferation and apoptosis of cervical cancer HeLa cells and explore its possible mechanism. The growth inhibitory effect was detected with MTT assay. After HeLa cells were treated with different concentrations (50, 100, 150 μmol•L⁻¹) of polydatin, MTT assay was used to detect the inhibitory effect of polydatin on proliferation of HeLa cells; Acridine orange/ethidium bromide staining was used for morphological changes in apoptotic HeLa cells； Annexin/propidium iodide staining was applied to detect HeLa cell apoptotic rate. In addition, flow cytometry was employed to analyze apoptosis and cell cycle distribution； RT-PCR and Western blot assay were used to detect PI3K, AKT, mTOR, and P70S6K mRNA and protein expression levels. The results showed that polydatin significantly inhibited HeLa cells proliferation in a dose-dependent manner. Polydatin can cause S phase arrest for HeLa cells, promote cell apoptosis and decrease the mRNA and protein expression levels of PI3K, AKT, mTOR and P70S6K. It indicated that polydatin could inhibit proliferation and induce apoptosis of cervical cancer HeLa cells, and the mechanism may be associated with inhibiting the PI3K/AKT/mTOR signaling pathway and suppressing downstream gene expression.
观察虎杖苷对宫颈癌HeLa细胞增殖和凋亡的影响，并探讨其可能的作用机制。采用MTT法检测其生长抑制作用。用不同浓度（50、100、150 μmol•L⁻¹）的虎杖苷处理HeLa细胞后，采用MTT法检测虎杖苷对HeLa细胞增殖的抑制作用；采用吖啶橙/溴化乙锭染色观察凋亡HeLa细胞的形态变化；采用膜联蛋白/碘化丙啶染色检测HeLa细胞凋亡率。此外，采用流式细胞术分析细胞凋亡及细胞周期分布；采用RT-PCR和Western blot法检测PI3K、AKT、mTOR和P70S6K的mRNA和蛋白表达水平。结果表明，虎杖苷能显著抑制HeLa细胞增殖，且呈剂量依赖性。虎杖苷可使HeLa细胞发生S期阻滞，促进细胞凋亡，并降低PI3K、AKT、mTOR和P70S6K的mRNA和蛋白表达水平。提示虎杖苷可抑制宫颈癌HeLa细胞的增殖并诱导其凋亡，其机制可能与抑制PI3K/AKT/mTOR信号通路及抑制下游基因表达有关。
