Bravo Durán Dolores Adriana, Silva Gómez Adriana Berenice, Gutiérrez Rosas Ana Coral, Trujillo Angélica
Laboratorio de Neurofisiología Experimental, Escuela de Biología, Universidad Autónoma de Puebla, Edificio 112A, Ciudad Universitaria, CP 72520 Puebla, Puebla, Mexico.
Laboratorio de Neurofisiología Experimental, Escuela de Biología, Universidad Autónoma de Puebla, Edificio 112A, Ciudad Universitaria, CP 72520 Puebla, Puebla, Mexico.
Brain Res. 2017 May 1;1662:102-109. doi: 10.1016/j.brainres.2017.03.001. Epub 2017 Mar 4.
Certain structures of the central nervous system (CNS) are morphologically and functionally related to the ovaries. Ovariectomy has been used to study the functional role of the ovaries in the CNS, as well as the role of the CNS on the reproductive system. In the present study, the effects of left and right hemi-ovariectomy on the morphology of pyramidal neurons from the CA1 and CA3 regions of the ventral hippocampus were studied. During the estrus phase, female Long-Evans rats underwent either left and right hemi-ovariectomies or left and right sham surgeries. Three estrous cycles later, the animals were sacrificed, and their brains were processed in Golgi-Cox stain and analyzed by the Sholl method to calculate the dendritic length of the CA1 and CA3 neurons of the left and right hemispheres. The results indicate that the dendritic lengths of the basilar and apical arbors of the CA1 neurons from the left hemisphere were shorter after both left and right hemi-ovariectomy, while the CA1 neurons from the right hemisphere were not affected by either procedure. However, the basilar dendritic arbors of the CA3 neurons from both hemispheres were affected by right hemi-ovariectomy. The spine density only decreased in the apical arbors in the CA3 neurons from the left hemisphere of rats that underwent right hemi-ovariectomy. This study's results indicate that hemi-ovariectomy in adult rats changes in the morphology of the CA1 and CA3 pyramidal neurons in the ventral hippocampus and that there are dimorphic responses between the hemispheres.
中枢神经系统（CNS）的某些结构在形态和功能上与卵巢相关。卵巢切除术已被用于研究卵巢在中枢神经系统中的功能作用，以及中枢神经系统对生殖系统的作用。在本研究中，研究了左右半卵巢切除术对腹侧海马CA1和CA3区锥体细胞形态的影响。在发情期，雌性Long-Evans大鼠接受左右半卵巢切除术或左右假手术。三个发情周期后，处死动物，对其大脑进行高尔基-考克斯染色处理，并采用肖尔方法进行分析，以计算左右半球CA1和CA3神经元的树突长度。结果表明，左右半卵巢切除术后，左半球CA1神经元基底和顶树突的长度均缩短，而右半球的CA1神经元不受任何一种手术的影响。然而，左右半球CA3神经元的基底树突受右半卵巢切除术的影响。仅在接受右半卵巢切除术的大鼠左半球CA3神经元的顶树突中，棘密度降低。本研究结果表明，成年大鼠半卵巢切除术会改变腹侧海马CA1和CA3锥体细胞的形态，且半球间存在二态性反应。
