López Mendoza Anayeli, Bravo Durán Dolores Adriana, Silva Gómez Adriana Berenice
Benemérita Universidad Autónoma de Puebla, Facultad de Ciencias Biológicas Laboratorio de Neurofisiología Experimental, Edificio BIO1, Ciudad Universitaria, CP 72570 Puebla, Puebla, Mexico.
Benemérita Universidad Autónoma de Puebla, Facultad de Ciencias Biológicas Laboratorio de Neurofisiología Experimental, Edificio BIO1, Ciudad Universitaria, CP 72570 Puebla, Puebla, Mexico.
Brain Res. 2018 Mar 1;1682:78-83. doi: 10.1016/j.brainres.2018.01.008. Epub 2018 Jan 11.
Studies have shown that changes in ovarian hormone concentrations promote natural fluctuations in the density of dendritic spines of hippocampal neurons in female Sprague-Dawley rats, without changes in dendritic length, throughout the estrous cycle. However, it is still unknown whether these fluctuations are present in other rat strains. Due to our interest in Wistar rats, the objective of the present study was to determine if there is natural dendritic remodeling in the female Wistar rat throughout the estrous cycle. This study analyzed the dendritic arborization of pyramidal neurons CA1 and CA3 of the dorsal hippocampus in each phase of the estrous cycle. We used the Golgi-Cox staining method and Sholl analysis to evaluate the dendritic length and density of dendritic spines. Our results showed that the dendritic length of the basilar and apical trees of CA1 neurons was longer in the metestrus phase. In CA3 neurons, only the apical dendritic trees showed longer dendritic length during metestrus. There was no variation in the density of dendritic spines in relation to any of the phases of the estrous cycle. Taken together, these results indicated that pyramidal neurons of the CA1 and CA3 regions of the dorsal hippocampus in the Wistar rat exhibited changes in dendritic length in the metestrus phase of the estrous cycle. Together, these data are important when considering the use of these organisms in behavioral studies.
研究表明，在雌性斯普拉格-道利大鼠的整个发情周期中，卵巢激素浓度的变化会促使海马神经元树突棘密度自然波动，而树突长度不变。然而，这些波动是否存在于其他大鼠品系中仍不清楚。由于我们对Wistar大鼠感兴趣，本研究的目的是确定雌性Wistar大鼠在整个发情周期中是否存在自然的树突重塑。本研究分析了发情周期各阶段背侧海马CA1和CA3区锥体神经元的树突分支情况。我们使用高尔基-考克斯染色法和肖尔分析来评估树突长度和树突棘密度。我们的结果表明，CA1神经元基底树和顶树的树突长度在动情后期较长。在CA3神经元中，只有顶树突在动情后期显示出较长的树突长度。发情周期各阶段的树突棘密度没有变化。综上所述，这些结果表明，Wistar大鼠背侧海马CA1和CA3区的锥体神经元在发情周期的动情后期表现出树突长度的变化。总之，在考虑将这些生物体用于行为研究时，这些数据很重要。
