Gu Li-Xia, Wen Zheng-Shun, Xiang Xing-Wei, Ma Li, Wang Xiao-Bo, Ma Jian-Yin, Qu You-Le
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; School of Marine Science and Technology, Zhejiang Ocean University, Zhejiang 316022, China.
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
Int J Biol Macromol. 2017 Jun;99:570-577. doi: 10.1016/j.ijbiomac.2017.03.008. Epub 2017 Mar 4.
Seleno-polysaccharides possess a variety of biological activities. In the present study, we further investigated the immunomodulatory effects of low molecular-weight seleno-aminopolysaccharides (LSA) in intestinal porcine epithelial cells (IPEC-1), and the molecular mechanisms of these effects. Analysis by ELISAs revealed that LSA could significantly increase the secretion of nitric oxide (NO), interleukin- 6 (IL-6), interleukin- 10 (IL-10), and tumor necrosis factor alpha (TNF-α). Moreover, LSA dramatically increased the gene expression levels of TNF-α, IL-6, IL-10, and iNOS in IPEC-1 cells, as determined by qRT-PCR. Western blot analysis further determined that LSA promotes inhibitor kappa B α (IĸBα), nuclear factor- kappa B (NF-κB) p65 phosphorylation. Taken together, these findings suggested that LSA has immunomodulatory activity on IPEC-1 cells, and its mechanism may be related to activation of the NF-ĸB signaling pathway.
硒多糖具有多种生物活性。在本研究中，我们进一步研究了低分子量硒氨基多糖（LSA）对猪肠道上皮细胞（IPEC-1）的免疫调节作用及其作用的分子机制。酶联免疫吸附测定（ELISA）分析表明，LSA可显著增加一氧化氮（NO）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）的分泌。此外，通过定量逆转录聚合酶链反应（qRT-PCR）测定，LSA显著提高了IPEC-1细胞中TNF-α、IL-6、IL-10和诱导型一氧化氮合酶（iNOS）的基因表达水平。蛋白质免疫印迹分析进一步确定，LSA促进抑制蛋白κBα（IκBα）、核因子κB（NF-κB）p65磷酸化。综上所述，这些发现表明LSA对IPEC-1细胞具有免疫调节活性，其机制可能与NF-κB信号通路的激活有关。
