Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; School of Marine Science and Technology, Zhejiang Ocean University, Zhejiang 316022, China.
Int J Biol Macromol. 2019 Feb 15;123:1278-1288. doi: 10.1016/j.ijbiomac.2018.10.099. Epub 2018 Oct 17.
Low molecular-weight seleno-aminopolysaccharides (LSA) have been shown to possess a variety of biological activities in vitro. In the present study, we further investigated the immunomodulatory effect of LSA on immunosuppressive mice induced by cyclophosphamide (CPA) and its molecular mechanism. The results demonstrated that LSA could significantly increase spleen and thymus indices, proliferation of splenic lymphocyte, the secretion of cytokines (IL-2, IL-4, IL-10 and INF-γ) of serum and ileum, and secretory immunoglobulin A (sIgA) content of small intestine. LSA dramatically improved the gene expression levels of IL-2, IL-4, IL-10 and INF-γ in small intestine by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Furthermore, our data indicated that LSA could significantly increase the gene expression levels of IL-1β and iNOS in RAW264.7 cells. LSA was further shown to remarkably promote inhibitor kappa Bα (IκBα) and nuclear factor-kappa B (NF-κB) p65 phosphorylation with western blot analysis. Taken together, these findings suggest that LSA has immunomodulatory activity on immunosuppressive mice and macrophage RAW264.7 cells, and its mechanism may be related to activation of NF-κB signaling pathway.
低相对分子质量硒代氨基多糖（LSA）已被证明在体外具有多种生物学活性。在本研究中，我们进一步研究了 LSA 对环磷酰胺（CPA）诱导的免疫抑制小鼠的免疫调节作用及其分子机制。结果表明，LSA 可显著增加脾和胸腺指数、脾淋巴细胞增殖、血清和回肠细胞因子（IL-2、IL-4、IL-10 和 INF-γ）的分泌以及小肠分泌型免疫球蛋白 A（sIgA）含量。实时荧光定量逆转录聚合酶链反应（qRT-PCR）显示，LSA 可显著提高小肠中 IL-2、IL-4、IL-10 和 INF-γ的基因表达水平。此外，我们的数据表明，LSA 可显著增加 RAW264.7 细胞中 IL-1β和 iNOS 的基因表达水平。Western blot 分析进一步表明，LSA 可显著促进抑制因子 kappa Bα（IκBα）和核因子 kappa B（NF-κB）p65 的磷酸化。综上所述，这些发现表明 LSA 对免疫抑制小鼠和巨噬细胞 RAW264.7 具有免疫调节活性，其机制可能与 NF-κB 信号通路的激活有关。
