基于 NMR 的代谢组学在高雄港沉积物环境评估中的应用——以海洋端足目动物（Hyalella azteca）为例。
NMR-based metabolomics for the environmental assessment of Kaohsiung Harbor sediments exemplified by a marine amphipod (Hyalella azteca).
机构信息
Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan.
Department of Marine Environmental Engineering, National Kaohsiung Marine University, Kaohsiung, Taiwan; Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan.
出版信息
Mar Pollut Bull. 2017 Nov 30;124(2):714-724. doi: 10.1016/j.marpolbul.2017.02.067. Epub 2017 Mar 6.
Inflow of wastewater from upstream causes a large flux of pollutants to enter Kaohsiung Harbor in Taiwan daily. To reveal the ecological risk posed by Kaohsiung Harbor sediments, an ecological metabolomic approach was employed to investigate environmental factors pertinent to the physiological regulation of the marine amphipod Hyalella azteca. The amphipods were exposed to sediments collected from different stream inlets of the Love River (LR), Canon River (CR), Jen-Gen River (JR), and Salt River (SR). Harbor entrance 1 (E1) was selected as a reference site. After 10-day exposure, metabolomic analysis of the Hyalella azteca revealed differences between two groups: {E1, LR, CR} and {JR, SR}. The metabolic pathways identified in the two groups of amphipods were significantly different. The results demonstrated that NMR-based metabolomics can be effectively used to characterize metabolic response related to sediment from polluted areas.
上游污水的流入导致大量污染物每天进入台湾高雄港。为了揭示高雄港沉积物带来的生态风险，采用生态代谢组学方法研究了与海洋桡足类 Hyalella azteca 生理调节相关的环境因素。将这些桡足类暴露于从爱河 (LR)、典宝溪 (CR)、后劲溪 (JR) 和盐埕溪 (SR) 的不同溪流入口采集的沉积物中。港口入口 1 (E1) 被选为参考点。经过 10 天的暴露，对 Hyalella azteca 的代谢组学分析显示两组之间存在差异：{E1、LR、CR}和{JR、SR}。两组桡足类动物鉴定出的代谢途径有显著差异。结果表明，基于 NMR 的代谢组学可有效用于表征与污染地区沉积物相关的代谢反应。
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