Shang Anquan, Yang Man, Shen Fujun, Wang Jun, Wei Jun, Wang Weiwei, Lu Wenying, Wang Chunbin, Wang Chunlei
Cell Physiol Biochem. 2017;41(3):1179-1188. doi: 10.1159/000464379. Epub 2017 Mar 6.
Bladder cancer is of compelling morbidity and mortality due to its high recurrence rate. Little development has been made in the last decades in the therapy methods. Thus, the mechanism of its growth and invasiveness involving novel molecular targets are needed.
Our research objective is to confirm the hypothesis that miR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells.
The expression levels of miR-1-3p and SFRP1 were evaluated using RT-qPCR in bladder cancer tissues and cells as well as in normal tissues and cells. J82 cell lines were selected as experiment subjects due to their low expression levels of miR-1-3p. Plasmids carrying miR-1-3p mimics, miR-1-3p inhibitors and SFRP1 were transfected into the J82 cell lines. Subsequently, the protein expression of SFRP1 was detected using Western Blot analysis, and cell proliferation, apoptosis, invasion and migration ability was measured using MTT, the flow cytometry, the Transwell test and wound healing assays, respectively Results: Bladder cancer tissues and cells exhibited significant decrease in the expression of miR-1-3p and SFRP1 compared to normal tissues and cells, and human bladder cancer cell line J82 exhibited the most significant decrease in these expressions (P < 0.05). MiR-1-3p up-regulates SFRP1 expression in bladder cancer cells, and the over-expression of miR-1-3p can suppress the proliferation, invasion and migration ability of bladder cancer cells. This mechanism is similar to the effect of SFRP1 over-expression on bladder cancer cells.
MiR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells by up-regulating SFRP1 expression.
膀胱癌因其高复发率而具有令人瞩目的发病率和死亡率。在过去几十年中，其治疗方法进展甚微。因此，需要了解其生长和侵袭机制中涉及的新分子靶点。
我们的研究目的是证实miR-1-3p抑制膀胱癌细胞增殖、侵袭和迁移的假说。
采用RT-qPCR评估膀胱癌组织和细胞以及正常组织和细胞中miR-1-3p和SFRP1的表达水平。由于J82细胞系中miR-1-3p表达水平较低，故选择其作为实验对象。将携带miR-1-3p模拟物、miR-1-3p抑制剂和SFRP1的质粒转染至J82细胞系。随后，采用蛋白质免疫印迹分析检测SFRP1的蛋白表达，分别采用MTT法、流式细胞术、Transwell试验和伤口愈合试验检测细胞增殖、凋亡、侵袭和迁移能力。结果：与正常组织和细胞相比，膀胱癌组织和细胞中miR-1-3p和SFRP1的表达显著降低，人膀胱癌细胞系J82在这些表达方面下降最为显著（P < 0.05）。miR-1-3p上调膀胱癌细胞中SFRP1的表达，miR-1-3p的过表达可抑制膀胱癌细胞的增殖、侵袭和迁移能力。该机制与SFRP1过表达对膀胱癌细胞的作用相似。
miR-1-3p通过上调SFRP1表达抑制膀胱癌细胞的增殖、侵袭和迁移。
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