Al Ahmed Hala Abdel, Nada Ola
Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Department of Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Cancer Biomark. 2017;19(1):21-26. doi: 10.3233/CBM-160196.
Many researches aiming to explore the pathogenesis of lung cancer have extensively studied the molecular alteration in such disease.
In the present study we measured the blood E2F3 mRNA using real-time RT-PCR technique in order to evaluate its clinical significance in early diagnosis and monitoring of lung cancer.
This case-control study included 50 lung cancer patients, 20 patients with benign lung diseases and 20 healthy controls. Relative quantification of blood E2F3 mRNA was done by real-time RT-PCR.
Blood E2F3 mRNA levels were significantly higher in lung cancer patients when compared to either patients with benign lung diseases or healthy subjects. This elevation was significant in those with metastatic lung cancer as compared to those with localized lung cancer. At a cutoff^{(2-Δ Δ CT)} 1.5, blood E2F3 mRNA was able to distinguish malignant from benign lung conditions with a diagnostic sensitivity of 100%; while at a cutoff^{(2-Δ Δ CT)} 5.3, blood E2F3 mRNA discriminated localized from metastatic lung cancer with a sensitivity of 93.6%.
Blood E2F3 mRNA is a sensitive diagnostic marker in lung cancer; moreover, it is a promising prognostic marker capable of efficiently discriminating early from late stages of the disease.
许多旨在探索肺癌发病机制的研究广泛地研究了该疾病中的分子改变。
在本研究中，我们使用实时逆转录聚合酶链反应（RT-PCR）技术检测血液中E2F3信使核糖核酸（mRNA），以评估其在肺癌早期诊断和监测中的临床意义。
本病例对照研究纳入50例肺癌患者、20例良性肺疾病患者和20名健康对照者。通过实时RT-PCR对血液中E2F3 mRNA进行相对定量分析。
与良性肺疾病患者或健康受试者相比，肺癌患者血液中E2F3 mRNA水平显著更高。与局限性肺癌患者相比，转移性肺癌患者的这种升高更为显著。在截断值（2-ΔΔCT）为1.5时，血液E2F3 mRNA能够以100%的诊断敏感性区分恶性与良性肺部疾病；而在截断值（2-ΔΔCT）为5.3时，血液E2F3 mRNA以93.6%的敏感性区分局限性与转移性肺癌。
血液E2F3 mRNA是肺癌的一种敏感诊断标志物；此外，它是一种有前景的预后标志物，能够有效区分疾病的早期和晚期。
