Cullin1与基质金属蛋白酶-2表达在结直肠癌诊断和预后中的协同作用
Synergistic efficacy of Cullin1 and MMP-2 expressions in diagnosis and prognosis of colorectal cancer.
作者信息
Deng Jianliang, Chen Wenjiao, Du Yuan, Wang Weiming, Zhang Guoqiang, Tang Yuehua, Qian Zhangjun, Xu Ping, Cao Zhihong, Zhou Yan
机构信息
Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
Department of Pathology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
出版信息
Cancer Biomark. 2017;19(1):57-64. doi: 10.3233/CBM-160341.
BACKGROUND
Cullin1 and MMP-2 have been identified as important markers in various cancers, but their roles in colorectal cancer (CRC) have remained to be discovered. The aim of this study was to investigate the expression pattern and significance of Cullin1 and MMP-2 in CRC.
METHODS
A total of 470 CRC patients were enrolled. Archival paraffin-embedded CRC tissue samples were used to generate tissue microarray blocks, which were immunohistochemically stained for Cullin1 and MMP-2. Prognostic and predictive role of Cullin1 and MMP-2 expression was evaluated by univariate and multivariate analysis, respectively. Values of p < 0.05 were considered statistically significant.
RESULTS
Cullin1 and MMP-2 protein levels were significantly upregulated in CRC tissues compared with adjacent noncancerous tissues. High tumoral Cullin1 or MMP-2 expression significantly correlated with shorter overall survival (OS), as well as with clinicopathologic characteristics in patients. Multivariate regression analysis showed that high Cullin1 and MMP-2 expressions, separately and together, were independent negative markers of OS.
CONCLUSION
Cullin1 and MMP-2 expressions could be novel diagnostic and prognostic markers for CRC patients.
背景
Cullin1和基质金属蛋白酶-2（MMP-2）已被确定为多种癌症中的重要标志物，但它们在结直肠癌（CRC）中的作用仍有待发现。本研究的目的是探讨Cullin1和MMP-2在结直肠癌中的表达模式及其意义。
方法
共纳入470例结直肠癌患者。采用存档的石蜡包埋结直肠癌组织样本制作组织芯片块，对其进行Cullin1和MMP-2的免疫组织化学染色。分别通过单因素和多因素分析评估Cullin1和MMP-2表达的预后和预测作用。p<0.05的值被认为具有统计学意义。
结果
与相邻的非癌组织相比，结直肠癌组织中Cullin1和MMP-2蛋白水平显著上调。肿瘤组织中Cullin1或MMP-2高表达与患者较短的总生存期（OS）以及临床病理特征显著相关。多因素回归分析表明，Cullin1和MMP-2的高表达单独或共同都是总生存期的独立负性标志物。
结论
Cullin1和MMP-2的表达可能是结直肠癌患者新的诊断和预后标志物。
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