基于双色贝塞尔光束的细胞分辨率多重荧光寿命成像断层扫描
Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.
作者信息
Xu Dongli, Zhou Weibin, Peng Leilei
机构信息
College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, MI 48109, USA.
出版信息
Biomed Opt Express. 2017 Jan 4;8(2):570-578. doi: 10.1364/BOE.8.000570. eCollection 2017 Feb 1.
Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.
傅里叶复用荧光寿命成像（FmFLIM）断层扫描技术能够对整个胚胎进行复用三维寿命成像。在我们之前的FmFLIM系统中，由于空间分辨率与成像深度之间的权衡，空间分辨率被限制在25μm。为了实现对厚标本的细胞分辨率成像，我们构建了一个双色贝塞尔光束断层扫描系统。结合FmFLIM，贝塞尔FmFLIM断层扫描系统能够在多个激发-发射通道上以2.8μm的细胞分辨率进行并行三维寿命成像。通过对双标记转基因斑马鱼胚胎的三维寿命成像，展示了贝塞尔FmFLIM断层扫描系统的成像能力。
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