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Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.

作者信息

Xu Dongli, Zhou Weibin, Peng Leilei

机构信息

College of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.

Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, MI 48109, USA.

出版信息

Biomed Opt Express. 2017 Jan 4;8(2):570-578. doi: 10.1364/BOE.8.000570. eCollection 2017 Feb 1.

DOI:10.1364/BOE.8.000570
PMID:28270968
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5330577/
Abstract

Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.

摘要

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引用本文的文献

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本文引用的文献

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