傅里叶复用荧光寿命成像。

Fourier Multiplexed Fluorescence Lifetime Imaging.

机构信息

Wyant College of Optical Science, University of Arizona, Tucson, AZ, USA.

出版信息

Methods Mol Biol. 2021;2350:157-172. doi: 10.1007/978-1-0716-1593-5_11.

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a widely used functional imaging method in bioscience. Fourier multiplexed FLIM (FmFLIM), a frequency-domain lifetime measurement method, explores the principle of Fourier (frequency) multiplexing to achieve parallel lifetime detection on multiple fluorescence labels. Combining FmFLIM with a confocal scanning microscope allows multiplexed 3D lifetime imaging of cells and tissues. FmFLIM can also be integrated with the scanning laser tomography imaging method to perform 3D multiplex lifetime imaging of whole embryos and thick tissues.

摘要

荧光寿命成像显微镜（FLIM）是生物科学中广泛应用的一种功能成像方法。傅里叶频分多路复用荧光寿命成像（FmFLIM）是一种频域寿命测量方法，它利用傅里叶（频率）多路复用的原理，实现了多个荧光标记物的并行寿命检测。将 FmFLIM 与共焦扫描显微镜相结合，可以对细胞和组织进行多维寿命成像。FmFLIM 还可以与扫描激光层析成像方法集成，对整个胚胎和厚组织进行多维寿命成像。