S期开始时合成的人类DNA序列的特征分析。

Tribioli C, Biamonti G, Giacca M, Colonna M, Riva S, Falaschi A

Istituto di Genetica Biochimica ed Evoluzionistica del CNR, Pavia, Italy.

Nucleic Acids Res. 1987 Dec 23;15(24):10211-32. doi: 10.1093/nar/15.24.10211.

We developed a method of enrichment for DNA replicated at the onset of S-phase in synchronized human HL60 cells. About 200 such sequences were cloned. The analysis of this selected DNA sample showed that: 1) the cloned DNA fragments derive from a limited number (750-1500) of replicons; 2) there is no extensive homology between different DNA fragments; 3) they are not significantly enriched in highly repeated sequences; 4) they are enriched in snap-back (Cot = o) DNA. The sequence of the longest fragment revealed the presence of numerous signals collected in a few hundred nucleotides: 1) homology with the origin of replication of human Papovaviruses usually associated with potential stem-loop structures; 2) binding sites for known transcription factors and for another nuclear factor; 3) potential binding sites for the chromosome "scaffold".

我们开发了一种在同步化的人HL60细胞中富集S期开始时复制的DNA的方法。克隆了约200个这样的序列。对这个选定的DNA样本的分析表明：1）克隆的DNA片段来自有限数量（750 - 1500）的复制子；2）不同DNA片段之间没有广泛的同源性；3）它们在高度重复序列中没有显著富集；4）它们在回折（Cot = o）DNA中富集。最长片段的序列显示在几百个核苷酸中存在大量信号：1）与人乳头多瘤病毒复制起点的同源性，通常与潜在的茎环结构相关；2）已知转录因子和另一种核因子的结合位点；3）染色体“支架”的潜在结合位点。