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[从小鼠间期染色体的玫瑰花结样结构(基本染色粒)的蛋白质核心中分离出的DNA的核苷酸序列]

[Nucleotide sequence of DNA isolated from protein cores of rosette-like structures (elementary chromomeres) of mouse interphase chromosomes].

作者信息

Glazkov M V, Poltaraus A B, Lebedeva I A

出版信息

Genetika. 1994 Sep;30(9):1146-54.

PMID:7545967
Abstract

Data are presented on the primary nucleotide sequence of 15 DNA fragments cloned from protein cores of rosette-like structures of mouse interphase chromosomes. According to data of a computer analysis, these DNA fragments represent protein-noncoding regions of interphase chromosomes, contain nucleotide sequences causing DNA strand bending, and contain a large amount of cis-regulatory transcriptional elements and sites closely related to sites of DNA topoisomerase II cleavage. Five clones demonstrated partial length homology with satellite DNA, short and long interspersed sequences, and extrachromosomal DNA from mouse thymocytes. Six clones showed homology with sites of initiation of replication of viruses, yeast, and mice. Six clones were found to contain consensus sequences for non-homologous recombination in somatic cells and six clones contained consensus sequences of human VTR1.1 loci.

摘要

本文展示了从小鼠间期染色体玫瑰花结样结构的蛋白质核心克隆的15个DNA片段的一级核苷酸序列数据。根据计算机分析数据,这些DNA片段代表间期染色体的蛋白质非编码区,包含导致DNA链弯曲的核苷酸序列,并且含有大量顺式调控转录元件以及与DNA拓扑异构酶II切割位点密切相关的位点。五个克隆与卫星DNA、短散在序列和长散在序列以及来自小鼠胸腺细胞的染色体外DNA表现出部分长度同源性。六个克隆与病毒、酵母和小鼠的复制起始位点具有同源性。发现六个克隆包含体细胞中非同源重组的共有序列,六个克隆包含人类VTR1.1基因座的共有序列。

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