Silva Alessandra Dutra da, Maraschin Bruna Jalfim, Laureano Natalia Koerich, Daroit Natália, Brochier Fernanda, Bündrich Leonardo, Visioli Fernanda, Rados Pantelis Varvaki
Universidade Luterana do Brasil, Department of Oral Pathology, Canoas, RS Brazil.
Universidade Federal do Rio Grande do Sul - UFRGS, Department of Oral Pathology, Porto Alegre, RS, Brazil.
Braz Oral Res. 2017 Mar 6;31:e19. doi: 10.1590/1807-3107BOR-2017.vol31.0019.
To assess the immunocytochemical and immunohistochemical correlation of adhesion (E-cadherin) and cell differentiation (involucrin) molecules in oral leukoplakia and oral squamous cell carcinoma. Cytological samples and biopsies were obtained from male and female patients aged over 30 years with oral leukoplakia (n = 30) and oral squamous cell carcinoma (n = 22). Cell scrapings and the biopsy were performed at the site of the lesion and histological slides were prepared for the immunocytochemical analysis of exfoliated oral mucosal cells and for the immunohistochemical analysis of biopsy tissues using E-cadherin and involucrin. Spearman's correlation and kappa coefficients were used to assess the correlation and level of agreement between the techniques. Immunostaining for E-cadherin and involucrin by both techniques was similar in the superficial layers of the histological sections compared with cell scrapings. However, there was no statistical correlation and agreement regarding the immunocytochemical and immunohistochemical expression of E-cadherin and involucrin in oral leukoplakia (R = 0.01, p = 0.958) (Kappa = 0.017, p = 0.92) or in oral squamous cell carcinoma (R = 0.26, p = 0.206) (Kappa = 0.36, p = 0.07). The immunoexpression of E-cadherin and involucrin in tissues is consistent with the expression patterns observed in exfoliated oral mucosal cells, despite the lack of a statistically significant correlation. There is an association of the histopathological characteristics of leukoplakia with the expression E-cadherin and of the microscopic aspects of oral squamous cell carcinoma with immunohistochemical expression of involucrin.
评估口腔白斑和口腔鳞状细胞癌中黏附分子（E-钙黏蛋白）和细胞分化分子（内披蛋白）的免疫细胞化学与免疫组织化学相关性。从年龄超过30岁的口腔白斑患者（n = 30）和口腔鳞状细胞癌患者（n = 22）中获取细胞学样本和活检组织，男女患者均有。在病变部位进行细胞刮取和活检，并制备组织学切片，用于对脱落的口腔黏膜细胞进行免疫细胞化学分析以及使用E-钙黏蛋白和内披蛋白对活检组织进行免疫组织化学分析。采用Spearman相关性分析和kappa系数评估两种技术之间的相关性和一致性水平。与细胞刮取相比，两种技术对组织学切片表层的E-钙黏蛋白和内披蛋白进行免疫染色的结果相似。然而，在口腔白斑中，E-钙黏蛋白和内披蛋白的免疫细胞化学和免疫组织化学表达之间不存在统计学相关性和一致性（R = 0.01，p = 0.958）（Kappa = 0.017，p = 0.92），在口腔鳞状细胞癌中也是如此（R = 0.26，p = 0.206）（Kappa = 0.36，p = 0.07）。尽管缺乏统计学上的显著相关性，但组织中E-钙黏蛋白和内披蛋白的免疫表达与脱落的口腔黏膜细胞中观察到的表达模式一致。白斑的组织病理学特征与E-钙黏蛋白的表达相关，口腔鳞状细胞癌的微观特征与内披蛋白的免疫组织化学表达相关。
