Sharada P, Swaminathan Uma, Nagamalini B R, Kumar K Vinod, Ashwini B K, Lavanya Vln
Department of Oral and Maxillofacial Pathology, AECS Maaruti College of Dental Sciences and Research Centre, Bengaluru, Karnataka, India.
J Oral Maxillofac Pathol. 2018 Jan-Apr;22(1):40-47. doi: 10.4103/jomfp.JOMFP_13_18.
Reduced E-cadherin expression and increased VEGF expression is known to be involved in tissue growth and transformation of Oral Potentially Malignant Disorders (OPMDs) and has been correlated with their differing histological grades in numerous studies.
To evaluate Immunohistochemical (IHC) expression of both E-cadherin and VEGF in predicting the malignant transformation potential of common OPMDs.
Ten cases each of Normal Oral mucosa (NOM), Mild Oral Epithelial Dysplasia (OED), Moderate OED, Severe OED, Oral Submucous Fibrosis, (OSMF) and Oral Squamous Cell Carcinoma (OSCC) were stained and evaluated for the expression of Ecadherin and VEGF. Quick score (QS) for expression intensity in all epithelial layers was calculated for both markers and results statistically analysed using Kruskal -Wallis ANOVA and Mann-Whitney "U" test.
E-cadherin expression was continuous and membranous in all the layers of NOM and reduced with progressing grades of OED to OSCC. In OSMF, expression was intermediate between moderate and severe OED. VEGF expression increased as the disease progressed from normal to increasing grades of OED to malignancy. In OSMF, expression was similar to that in mild OED. VEGF, E-cadherin expression for basal and parabasilar cells showed a strong statistically significant negative correlation in NOM. A very strong statistically significant positive correlation with perfect monotonic relation was noted in superficial cells in severe OED group and OSCC group.
E-Cadherin and VEGF could be used as combination markers to predict the potential risk for malignant transformation in OEDs.
已知E-钙黏蛋白表达降低和血管内皮生长因子（VEGF）表达增加与口腔潜在恶性疾病（OPMDs）的组织生长和转化有关，并且在众多研究中已与其不同的组织学分级相关。
评估E-钙黏蛋白和VEGF的免疫组织化学（IHC）表达，以预测常见OPMDs的恶性转化潜能。
对正常口腔黏膜（NOM）、轻度口腔上皮发育异常（OED）、中度OED、重度OED、口腔黏膜下纤维化（OSMF）和口腔鳞状细胞癌（OSCC）各10例进行染色，并评估E-钙黏蛋白和VEGF的表达。计算两种标志物在所有上皮层中的表达强度快速评分（QS），并使用Kruskal-Wallis方差分析和Mann-Whitney “U”检验对结果进行统计学分析。
E-钙黏蛋白在NOM的所有层中呈连续性膜表达，随着OED至OSCC分级的进展而降低。在OSMF中，表达介于中度和重度OED之间。随着疾病从正常进展至OED分级增加再到恶性，VEGF表达增加。在OSMF中，表达与轻度OED相似。在NOM中，基底细胞和副基底细胞的VEGF、E-钙黏蛋白表达呈强统计学显著负相关。在重度OED组和OSCC组的表层细胞中，观察到具有完美单调关系的非常强的统计学显著正相关。
E-钙黏蛋白和VEGF可作为联合标志物来预测OEDs中恶性转化的潜在风险。
