金属氧化物纳米颗粒暴露工人的全球 DNA 甲基化和氧化应激生物标志物。
Global DNA methylation and oxidative stress biomarkers in workers exposed to metal oxide nanoparticles.
机构信息
National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Miaoli County, Taiwan.
Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan.
出版信息
J Hazard Mater. 2017 Jun 5;331:329-335. doi: 10.1016/j.jhazmat.2017.02.042. Epub 2017 Feb 24.
This is the first study to assess global methylation, oxidative DNA damage, and lipid peroxidation in workers with occupational exposure to metal oxide nanomaterials (NMs). Urinary and white blood cell (WBC) 8-hydroxydeoxyguanosine (8-OHdG), and exhaled breath condensate (EBC) 8-isoprostane were measured as oxidative stress biomarkers. WBC global methylation was measured as an epigenetic alteration. Exposure to TiO, SiO and indium tin oxide (ITO) resulted in significantly higher oxidative biomarkers such as urinary 8-OHdG and EBC 8-isoprostane. However, significantly higher WBC 8-OHdG and lower global methylation were only observed in ITO handling workers. Significant positive correlations were noted between WBC and urinary 8-OHdG (Spearman correlation r=0.256, p=0.003). Furthermore, a significant negative correlation was found between WBC 8-OHdG and global methylation (r=-0.272, p=0.002). These results suggest that exposure to metal oxide NMs may lead to global methylation, DNA oxidative damage, and lipid peroxidation.
这是第一项评估职业性接触金属氧化物纳米材料（NMs）工人的全球甲基化、氧化 DNA 损伤和脂质过氧化的研究。尿和白细胞（WBC）8-羟基脱氧鸟苷（8-OHdG）和呼出气冷凝物（EBC）8-异前列腺素被作为氧化应激生物标志物进行测量。WBC 整体甲基化作为一种表观遗传改变进行测量。接触 TiO、SiO 和铟锡氧化物（ITO）导致尿 8-OHdG 和 EBC 8-异前列腺素等氧化生物标志物显著升高。然而，只有在处理 ITO 的工人中才观察到 WBC 8-OHdG 显著升高和整体甲基化水平显著降低。WBC 与尿 8-OHdG 之间存在显著正相关（Spearman 相关系数 r=0.256，p=0.003）。此外，还发现 WBC 8-OHdG 与整体甲基化之间存在显著负相关（r=-0.272，p=0.002）。这些结果表明，接触金属氧化物纳米材料可能导致整体甲基化、DNA 氧化损伤和脂质过氧化。
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