Narendrula-Kotha Ramya, Nkongolo Kabwe K
Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, Canada P3E 2C6.
Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario, Canada P3E 2C6; Department of Biology, Laurentian University, Sudbury, Ontario, Canada P3E 2C6.
Ecotoxicol Environ Saf. 2017 Jun;140:241-248. doi: 10.1016/j.ecoenv.2017.02.040. Epub 2017 Mar 8.
Metal and sulfur dioxide (SO) contaminations in Northern Ontario (Canada), especially in the Greater Sudbury Region (GSR) caused by mining activities have resulted in severe environmental degradations. A long term restoration program has led to significant landscape changes and healthy ecosystems. The objective of this study was to assess variation in enzymatic activities and soil respiration in metal contaminated and reclaimed ecosystems. Soil analysis revealed that respiration rates were higher in metal contaminated limed soils (65ppm) compared to adjacent unlimed areas (35ppm). The respiration rates in metal contaminated sites (55ppm) were significantly lower compared to reference (metal-uncontaminated) areas (90ppm). β-glucosidase (BG), cellobiohydrolase (CBH), β-N-acetylglucosaminidase (NAGase), aryl sulfatase (AS), acid phosphatase (AP), alkaline phosphatase (AlP), glycine aminopeptidase (GAP), and leucine aminopeptidase (LAP) activities were significantly higher in limed compared to unlimed sites. Metal contamination significantly reduced the activities of these enzymes with the exception of LAP. An opposite trend was observed for peroxidase (PER) activity that was lower in limed compared to corresponding unlimed areas. Likewise, PER activity values were significantly lower in metal contaminated than in uncontaminated reference sites.
加拿大安大略省北部，尤其是大萨德伯里地区（GSR）的采矿活动造成的金属和二氧化硫（SO）污染，已导致严重的环境退化。一项长期恢复计划已带来显著的景观变化和健康的生态系统。本研究的目的是评估金属污染和复垦生态系统中酶活性和土壤呼吸的变化。土壤分析表明，与相邻的未加石灰的区域（35ppm）相比，金属污染的加石灰土壤（65ppm）中的呼吸速率更高。与参考（未受金属污染）区域（90ppm）相比，金属污染场地（55ppm）中的呼吸速率显著更低。与未加石灰的场地相比，加石灰场地中的β-葡萄糖苷酶（BG）、纤维二糖水解酶（CBH）、β-N-乙酰氨基葡萄糖苷酶（NAGase）、芳基硫酸酯酶（AS）、酸性磷酸酶（AP）、碱性磷酸酶（AlP）、甘氨酰氨基肽酶（GAP）和亮氨酸氨基肽酶（LAP）活性显著更高。除LAP外，金属污染显著降低了这些酶的活性。对于过氧化物酶（PER）活性，观察到相反的趋势，与相应的未加石灰区域相比，加石灰区域中的PER活性更低。同样，金属污染场地中的PER活性值显著低于未受污染的参考场地。
