Mohan Shikha, Gupta Damodar
Metabolic Cell Signaling Research, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization, Timarpur, Delhi, 110 054, India.
Metabolic Cell Signaling Research, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization, Timarpur, Delhi, 110 054, India.
Biomed Pharmacother. 2017 May;89:781-795. doi: 10.1016/j.biopha.2017.02.053. Epub 2017 Mar 6.
The root of Inula racemosa is known for its antifungal, hypolipdemic and antimicrobial properties in traditional Indian Ayurvedic and Chinese system of medicine. The biological efficacy of Inula species is mainly due to the presence sesquiterpene lactone (Isoalantolactone and Alantolactone), which are reported to be inducers of Nrf2 antioxidant pathway. The investigation of properties and efficacy of root extracts of I. racemosa and their comparison was done with a view to find most efficacious extract for use at cellular level (both normal and transformed). In the present study different extracts of root of I. racemosa (aqueous, ethanolic, and 50% aqueous-ethanolic) were prepared and compared for their antioxidant potential, reducing capacity, polyphenol content and flavonoid content. Our investigations suggested that the aqueous extract possess highest antioxidant capacity and reducing potential. The polyphenol content was found to be highest in aqueous extract in comparison with other two extracts. However, all the three extracts showed less flavonoid content. Further, the preliminary phytochemical screening of all the extracts revealed the presence of terpenoids, phytosterols and glycosides. The TLC profile of ethanolic and 50% aqueous-ethanolic extracts showed the presence of alantolactone while aqueous extracts did not exhibit its strong presence. This warrants the need of more stringent techniques for characterization of aqueous extract in future. The in vitro cell based toxicity assays revealed that the aqueous extract was less toxic to kidneys cells while ethanolic extract was toxic to cells even at low concentrations. Hence, the current investigations showed better efficacy of the aqueous extract with respect to other extracts and found to be promising for its future application at in vitro levels.
在传统的印度阿育吠陀医学和中国医学体系中，总状土木香的根以其抗真菌、降血脂和抗菌特性而闻名。土木香属植物的生物学功效主要归因于倍半萜内酯（异土木香内酯和土木香内酯）的存在，据报道它们是Nrf2抗氧化途径的诱导剂。对总状土木香根提取物的性质和功效进行研究并进行比较，旨在找到在细胞水平（正常细胞和转化细胞）上最有效的提取物。在本研究中，制备了总状土木香根的不同提取物（水提取物、乙醇提取物和50%水-乙醇提取物），并比较了它们的抗氧化潜力、还原能力、多酚含量和黄酮含量。我们的研究表明，水提取物具有最高的抗氧化能力和还原潜力。与其他两种提取物相比，水提取物中的多酚含量最高。然而，所有三种提取物的黄酮含量都较低。此外，对所有提取物的初步植物化学筛选显示存在萜类化合物、植物甾醇和糖苷。乙醇提取物和50%水-乙醇提取物的薄层色谱图谱显示存在土木香内酯，而水提取物中未显示其大量存在。这就需要在未来采用更严格的技术来表征水提取物。体外细胞毒性试验表明，水提取物对肾细胞的毒性较小，而乙醇提取物即使在低浓度下对细胞也有毒性。因此，目前的研究表明，水提取物相对于其他提取物具有更好的功效，并且发现在体外水平上具有未来应用前景。
