School of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144411, India.
Department of Zoology, Institute of Sciences, Banaras Hindu University, Varanasi 221005, India.
Molecules. 2022 Dec 6;27(23):8629. doi: 10.3390/molecules27238629.
Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella bursa-pastoris (CBP), and Inula racemosa (IR). The total phenolic and flavonoid contents (TPC and TFC) were illustrated through colorimetric assays, while the antioxidant activity was investigated through DPPH and ABTS assays. The evaluation of phytochemicals by FT-IR of UD and CBP revealed high contents of aliphatic amines, while IR showed a major peak for ketones. The antioxidant activity, TPC and TFC were highest in the ethanol extract of UD, followed by CBP, and IR showed the lowest activity. All of the extracts revealed significant antioxidant capacities along a dosage gradient. Through a HPLC analysis at a wavelength of 280 nm, UD leaves demonstrated an intense peak of quercetin, and the peak for rutin was less intense. CBP (whole plant), instead, demonstrated a major yield of rutin, and a peak for quercetin was not observed in CBP. IR (rhizomes) showed both quercetin and rutin. All of the extracts were significantly cytotoxic to HepG2 cells after 48 h with the trend IR > UD > CBP. The outcomes of this study may be effective in the selection of specific plants as realistic sources of the bioactive components that might be useful in the nutraceutical progression and other biomedical efficacies.
植物来源的天然抗氧化剂在预防多种人类慢性疾病方面发挥了重要作用，并为药物治疗发现的研究人员提供了新的生物活性线索。这项工作旨在研究荨麻（UD）、荠菜（CBP）和旋覆花（IR）的民族药理学作用。通过比色法测定总酚和类黄酮含量（TPC 和 TFC），通过 DPPH 和 ABTS 测定法研究抗氧化活性。通过 FT-IR 对 UD 和 CBP 中的植物化学成分进行评估，结果表明它们含有丰富的脂肪胺，而 IR 则显示出酮类的主要峰。UD 乙醇提取物的抗氧化活性、TPC 和 TFC 最高，其次是 CBP，IR 的活性最低。所有提取物在剂量梯度上均表现出显著的抗氧化能力。通过在 280nm 波长处进行 HPLC 分析，UD 叶片显示出强烈的槲皮素峰，而芦丁的峰较弱。相反，CBP（全草）显示出大量芦丁，而 CBP 中未观察到槲皮素峰。IR（根茎）显示出槲皮素和芦丁。所有提取物在 48 小时后对 HepG2 细胞均具有显著的细胞毒性，IR > UD > CBP 的趋势。这项研究的结果可能有助于选择特定的植物作为生物活性成分的实际来源，这些成分可能对营养保健品的进展和其他生物医学疗效有用。
