Trinconi Cristiana T, Reimão Juliana Q, Bonano Vivian I, Espada Caroline R, Miguel Danilo C, Yokoyama-Yasunaka Jenicer K U, Uliana Silvia R B
Departamento de Parasitologia,Instituto de Ciências Biomédicas, Universidade de São Paulo,Av. Prof. Lineu Prestes 1374,05508-000 São Paulo,Brazil.
Parasitology. 2018 Apr;145(4):490-496. doi: 10.1017/S0031182017000130. Epub 2017 Mar 9.
The aims of the present work were to test the effect of tamoxifen administered topically and the therapeutic efficacy of tamoxifen and pentavalent antimonial combinations in an experimental model of cutaneous leishmaniasis. BALB/c mice infected with a luciferase expressing line of Leishmania amazonensis were treated with topical tamoxifen in two different formulations (ethanol or oil-free cream) as monotherapy or in co-administration with pentavalent antimonial. Treatment efficacy was evaluated by lesion size and parasite burden, quantified through luminescence, at the end of treatment and 4 weeks later. Topical tamoxifen, formulated in ethanol or as a cream, was shown to be effective. The interaction between tamoxifen and pentavalent antimonial was additive in vitro. Treatment with combined schemes containing tamoxifen and pentavalent antimonial was effective in reducing lesion size and parasite burden. Co-administration of tamoxifen and pentavalent antimonial was superior to monotherapy with antimonial.
本研究的目的是在皮肤利什曼病实验模型中测试局部应用他莫昔芬的效果以及他莫昔芬与五价锑联合用药的治疗效果。用表达荧光素酶的亚马逊利什曼原虫株感染的BALB/c小鼠，分别用两种不同剂型（乙醇或无油乳膏）的他莫昔芬进行单药治疗，或与五价锑联合给药。在治疗结束时及4周后，通过发光定量评估病变大小和寄生虫负荷，以此评价治疗效果。结果表明，以乙醇或乳膏形式配制的局部用他莫昔芬是有效的。他莫昔芬与五价锑在体外具有相加作用。含他莫昔芬和五价锑的联合方案治疗可有效减小病变大小并减轻寄生虫负荷。他莫昔芬与五价锑联合给药优于锑单药治疗。
