Li Cui, Zhang Yaoyao, Eremin Sergei A, Yakup Omar, Yao Gang, Zhang Xiaoying
College of Veterinary Medicine, Northwest A&F University, Yangling, 712100 Shaanxi, China.
Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia; Federal Research Center «Fundamentals of Biotechnology» of the Russian Academy of Sciences, 119071 Moscow, Russia.
Food Chem. 2017 Jul 15;227:48-54. doi: 10.1016/j.foodchem.2017.01.058. Epub 2017 Jan 16.
Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC of FPIA and ic-ELISA based anti-Gent IgY were 7.70±0.6μg/mL and 0.32±0.06μg/mL, respectively. The IC of FPIA and ic-ELISA based anti-Kana IgY were 7.97±0.9μg/mL and 0.15±0.01μg/mL. The limits of detection (LOD, IC) for FPIA based anti-Gent/Kana IgY were 0.17 and 0.007μg/mL, respectively. The LOD for ic-ELISA were both 0.001μg/mL. These results indicated that the ic-ELISA might more suitable for antibiotic residues detection than FPIA.
本研究的目的是表明基于IgY抗体的免疫测定法可用于检测动物源性食品中的抗生素残留。简而言之，使用庆大霉素（Gent）和卡那霉素（Kana）的完全抗原免疫蛋鸡以制备IgY抗体。然后，通过荧光偏振免疫分析（FPIA）和间接竞争酶联免疫吸附测定（ic-ELISA）评估这些抗体，以检测动物源性样品中的Gent/Kana。基于抗Gent IgY的FPIA和ic-ELISA的半数抑制浓度（IC）分别为7.70±0.6μg/mL和0.32±0.06μg/mL。基于抗Kana IgY的FPIA和ic-ELISA的IC分别为7.97±0.9μg/mL和0.15±0.01μg/mL。基于抗Gent/Kana IgY的FPIA的检测限（LOD，IC）分别为0.17和0.007μg/mL。ic-ELISA的LOD均为0.001μg/mL。这些结果表明，与FPIA相比，ic-ELISA可能更适合用于抗生素残留检测。
