Shamsi Mohammad, Zahedi Payam, Ghourchian Hedayatollah, Minaeian Sara
Nano-Biopolymers Research Laboratory, School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran.
Nano-Biopolymers Research Laboratory, School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran.
Int J Biol Macromol. 2017 Jun;99:433-442. doi: 10.1016/j.ijbiomac.2017.03.013. Epub 2017 Mar 6.
The aim of this work was to provide a microfluidic-aided fabrication of nanoparticles based on chitosan/poly(N-isopropylacrylamide-co-acrylic acid)/cellulose laurate [CS/P(NIPAAm-co-AAc/CL] blend for transdermal multidrug delivery applications. The scanning electron microscopy (SEM) and dynamic light scattering (DLS) results showed that the diameter sizes of samples were in the range from 200 to 300nm along with a narrow size distribution. Also, the CS-based nanoparticles containing tretinoin and clindamycin phosphate prepared using microfluidic technique exhibited a sustained control release of the drugs as well as minimum inhibitory and bactericidal concentrations compared to the samples fabricated via bulk mixing method. The thermal stability of the drugs loaded nanoparticles revealed a reduction in degradation for those fabricated by using microfluidic technique at 45°C for one month. Afterward, the in vivo assessments confirmed that by applying the microfluidically generated nanoparticles containing two drugs, a declined superficial reddening (erythema) and suitable transdermal permeation as well as residency were happened with respect to the those samples prepared via bulk mixing method and also the drugs solution alone. Finally, these CS-based nanoparticles showed sufficient potential used for transdermal multidrug delivery applications.
本研究旨在通过微流控辅助制备基于壳聚糖/聚（N-异丙基丙烯酰胺-co-丙烯酸）/月桂酸纤维素[CS/P（NIPAAm-co-AAc/CL）]共混物的纳米颗粒，用于经皮多药递送应用。扫描电子显微镜（SEM）和动态光散射（DLS）结果表明，样品的直径尺寸在200至300nm范围内，且尺寸分布狭窄。此外，与通过本体混合法制备的样品相比，使用微流控技术制备的含有维甲酸和磷酸克林霉素的基于CS的纳米颗粒表现出药物的持续控释以及最低抑菌和杀菌浓度。负载药物的纳米颗粒的热稳定性表明，通过微流控技术在45°C下制备一个月的纳米颗粒的降解有所减少。随后，体内评估证实，应用含有两种药物的微流控生成的纳米颗粒，相对于通过本体混合法制备的样品以及单独的药物溶液，表面发红（红斑）减少，经皮渗透和滞留合适。最后，这些基于CS的纳米颗粒显示出用于经皮多药递送应用的足够潜力。
