Tatsuta Takashi
Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Joseph-Stelzmann-Str. 26, 50674, Cologne, Germany.
Methods Mol Biol. 2017;1567:79-103. doi: 10.1007/978-1-4939-6824-4_7.
Lipids draw increasing attention of cell biologists because of the wide variety of functions beyond their role as building blocks of cellular membranes. Mitochondrial membranes possess characteristic lipid compositions that are intimately associated with mitochondrial architecture and activities. Therefore, quantitative assessment of lipids in isolated mitochondria is of importance for mitochondrial research. Here, I describe our workflow for quantitative analysis of glycerophospholipids in mitochondria with a focus on purification of pure mitochondrial fractions from yeast and cultured mammalian cells as well as improved settings for the analysis of cardiolipin by nano-electrospray ionization mass spectrometry.
脂质因其作为细胞膜结构单元之外的多种功能而日益受到细胞生物学家的关注。线粒体膜具有独特的脂质组成，这些脂质组成与线粒体的结构和活性密切相关。因此，对分离出线粒体中的脂质进行定量评估对于线粒体研究具有重要意义。在此，我描述了我们用于线粒体中甘油磷脂定量分析的工作流程，重点是从酵母和培养的哺乳动物细胞中纯化纯线粒体组分，以及通过纳米电喷雾电离质谱分析心磷脂的改进设置。
