Hamdan Islam J A, Claumarchirant Lorena, Garcia-Llatas Guadalupe, Alegría Amparo, Lagarda María Jesús
a Nutrition and Food Science Area, Faculty of Pharmacy , University of Valencia , Valencia , Spain.
Int J Food Sci Nutr. 2017 Sep;68(6):695-703. doi: 10.1080/09637486.2017.1287883. Epub 2017 Feb 16.
Sterols are components present in the fat fraction of infant formulas (IFs). Their characterization is therefore of interest, though there are no official reference methods for their analysis in these matrices.
To validate a gas chromatographic method with flame ionization detection for the determination of animal (cholesterol and desmosterol) and plant sterols (brassicasterol, campesterol, stigmasterol, β-sitosterol and sitostanol) found in IFs. All correlation coefficients obtained for the calibration curves of sterols studied were >0.99. Limits of detection (<1 μg/100 mL) and quantification (<4 μg/100 mL) are suitable for sterols determination in IFs. The within-assay precision ranged from 1.6% to 8.8%, while the between-assay precision was <10% for most of sterols. Accuracy was satisfactory and was calculated by recovery assays (ranging 93-108%). The analytical parameters obtained showed the suitability of the proposed method for the determination of sterols in IFs.
甾醇是婴儿配方奶粉（IFs）脂肪部分中的成分。因此，对其进行表征很有意义，尽管目前尚无针对这些基质中甾醇分析的官方参考方法。
验证一种带火焰离子化检测的气相色谱法，用于测定婴儿配方奶粉中存在的动物甾醇（胆固醇和胆甾烯醇）和植物甾醇（油菜甾醇、菜油甾醇、豆甾醇、β-谷甾醇和谷甾烷醇）。所研究的甾醇校准曲线获得的所有相关系数均>0.99。检测限（<1μg/100mL）和定量限（<4μg/100mL）适用于婴儿配方奶粉中甾醇的测定。批内精密度范围为1.6%至8.8%，而大多数甾醇的批间精密度<10%。准确度令人满意，通过回收率测定计算得出（范围为93 - 108%）。获得的分析参数表明所提出的方法适用于测定婴儿配方奶粉中的甾醇。
