Zou Qin, Cai Bin, Li Junfeng, Li Jidong, Li Yubao
a Research Center for Nano-Biomaterials, Analytical & Testing Center , Sichuan University , Chengdu , China.
b Department of Materials Science & Engineering , Chengdu University of Technology , Chengdu , China.
J Biomater Sci Polym Ed. 2017 May;28(7):601-615. doi: 10.1080/09205063.2017.1289036. Epub 2017 Feb 13.
We have developed tourmaline/chitosan (Tur/CS) composite films for wound healing applications. The characteristics of composite films were studied by optical microscope, infrared spectra and X-ray diffraction. Tur particles were uniformly distributed in the CS film and the crystal structure of CS was not remarkably changed except the decrease of crystallinity. The influence of Tur on wound healing applications was characterized by modulating Tur concentrations in the Tur/CS composite film prepared by loading Tur powder into CS matrix with different proportion (0, 1/40 and 1/10). Then L929 cells were co-cultured on the composite films to access the cytotoxicity in vitro. Tur concentrations strongly influenced cell process extension. Tur/CS composite film with 1/40 mass ratio could promote the cell adhesion and proliferation. Fewer and shorter processes were observed at high Tur density. When the composite films were transplanted on porcine full-thickness burn wounds, histological results demonstrated that the Tur/CS group with 1/40 mass ratio had a significantly higher number of newly-formed and mature blood vessels, and fastest regeneration of dermis. Based on the observed facts these films can be tailored for their potential utilization in wound healing and skin tissue engineering applications.
我们已开发出用于伤口愈合的电气石/壳聚糖（Tur/CS）复合膜。通过光学显微镜、红外光谱和X射线衍射研究了复合膜的特性。Tur颗粒均匀分布在CS膜中，除结晶度降低外，CS的晶体结构没有明显变化。通过将Tur粉末以不同比例（0、1/40和1/10）负载到CS基质中制备Tur/CS复合膜，调节Tur浓度来表征Tur对伤口愈合应用的影响。然后将L929细胞与复合膜共培养以评估体外细胞毒性。Tur浓度强烈影响细胞突起的延伸。质量比为1/40的Tur/CS复合膜可促进细胞黏附和增殖。在高Tur密度下观察到的细胞突起更少且更短。当将复合膜移植到猪全层烧伤伤口上时，组织学结果表明，质量比为1/40的Tur/CS组新生和成熟血管数量明显更多，真皮再生最快。基于观察到的这些事实，这些膜可根据其在伤口愈合和皮肤组织工程应用中的潜在用途进行定制。
