Advanced Rheology Institute, Department of Polymer Science and Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.
Macromol Biosci. 2021 Apr;21(4):e2000432. doi: 10.1002/mabi.202000432. Epub 2021 Feb 18.
A novel chitosan composite hydrogel by combining functionalized graphene oxide (CGO) is fabricated. The introduction of CGO significantly improves the mechanical property of CS hydrogel owing to the enhanced interaction between chitosan and CGO sheets. In comparison to the CS-GO composite hydrogel, the compressive stress of the CS-CGO composite hydrogel increases from 1.9 MPa at strain of 70.4% to 4.2 MPa at strain of 78.4%, the tensile stress and strain improve from 141.2 kPa and 134.6% to 300.2 kPa and 165.9%, respectively. An interconnected porous structure is formed in the CS-CGO composite hydrogel and the pore size decreases as the CGO loading increases, which is desirable in improving its mechanical property. Furthermore, the cytotoxicity tests indicate that the CS-CGO composite hydrogel possesses an excellent biocompatibility and can promote the adhesion and proliferation of fibroblasts. In vivo evaluation on full-thickness excision wounds in experimental rat shows that the CS-CGO composite hydrogel significantly accelerates wound healing, and the wound closure rate reaches up to 92.2% after 21 days. A feasible strategy to fabricate an enhanced chitosan composite hydrogel for application in wound healing is offered.
一种新型壳聚糖复合水凝胶是通过结合功能化氧化石墨烯（CGO）制备的。CGO 的引入显著提高了 CS 水凝胶的机械性能，这是由于壳聚糖和 CGO 片之间增强的相互作用。与 CS-GO 复合水凝胶相比，CS-CGO 复合水凝胶的压缩应力从应变 70.4%时的 1.9 MPa 增加到应变 78.4%时的 4.2 MPa，拉伸应力和应变从 141.2 kPa 和 134.6%增加到 300.2 kPa 和 165.9%，分别。CS-CGO 复合水凝胶中形成了相互连通的多孔结构，随着 CGO 负载的增加，孔径减小，这有利于提高其机械性能。此外，细胞毒性试验表明，CS-CGO 复合水凝胶具有优异的生物相容性，能促进成纤维细胞的黏附和增殖。在实验大鼠全层切除伤口的体内评价表明，CS-CGO 复合水凝胶能显著加速伤口愈合，在 21 天后伤口闭合率达到 92.2%。为应用于伤口愈合提供了一种制备增强壳聚糖复合水凝胶的可行策略。
