PG and Research Department of Chemistry, Islamiah College (Autonomous), Vaniyambadi 635 752, Tamil Nadu, India.
PG and Research Department of Chemistry, Islamiah College (Autonomous), Vaniyambadi 635 752, Tamil Nadu, India.
Int J Biol Macromol. 2020 Jun 1;152:873-883. doi: 10.1016/j.ijbiomac.2020.02.289. Epub 2020 Feb 26.
A biocomposite film composed of biopolymers chitosan (CS), gelatin (GE) and allantoin (AT) was fabricated by solution casting technique. The functional group interaction of the biocomposite films was inspected through the Attenuated total reflectance Fourier transform spectrometer (ATR-FTIR). The morphological changes and crystallinity of biocomposite films with varied ratios of chitosan/gelatin (CS/GE) and allantoin were examined under Scanning electron microscope (SEM) and X-ray diffractometer (XRD). The water-absorbing capacity was found enhanced by an increase in the chitosan ratio. The biocomposite films exhibit good antioxidant, anti-inflammatory properties. The biocomposite films also display enhanced stability with steady degradation under the PBS medium. The biocomposite films reveal improved antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). Blood compatibility studies explore the non-hemolytic nature. The in vitro cytotoxicity by MTT assay shows excellent biocompatibility. The fibroblast adhesion on the biocomposite film displays enhanced proliferation and viability. These significant biological properties of biocomposite film make it an appropriate candidate for wound dressing application.
采用溶液浇铸法制备了由生物聚合物壳聚糖（CS）、明胶（GE）和尿囊素（AT）组成的生物复合膜。通过衰减全反射傅里叶变换光谱仪（ATR-FTIR）检测了生物复合膜的官能团相互作用。通过扫描电子显微镜（SEM）和 X 射线衍射仪（XRD）研究了不同壳聚糖/明胶（CS/GE）和尿囊素比的生物复合膜的形貌变化和结晶度。吸水能力随着壳聚糖比例的增加而提高。生物复合膜表现出良好的抗氧化、抗炎性能。生物复合膜在 PBS 介质中也表现出良好的稳定性和稳定降解。生物复合膜对大肠杆菌（E.coli）和金黄色葡萄球菌（S.aureus）的抗菌活性得到提高。血液相容性研究探讨了非溶血性质。MTT 测定的体外细胞毒性显示出优异的生物相容性。成纤维细胞在生物复合膜上的黏附显示出增强的增殖和活力。生物复合膜具有这些显著的生物学性能，使其成为一种合适的伤口敷料应用候选材料。
