Czyzyk Adam, Filipowicz Dorota, Podfigurna Agnieszka, Ptas Paula, Piestrzynska Malgorzata, Smolarczyk Roman, Genazzani Andrea R, Meczekalski Blazej
a Department of Gynecological Endocrinology , Poznan University of Medical Sciences , Poznan , Poland.
b Students Scientific Association of the Department of Gynecological Endocrinology, Poznan University of Medical Sciences , Poznan , Poland.
Gynecol Endocrinol. 2017 May;33(5):413-417. doi: 10.1080/09513590.2017.1290073. Epub 2017 Feb 28.
Premature ovarian insufficiency (POI) is defined as a cessation of function of ovaries in women younger than 40 years old. Brain-derived neurotrophic factor (BDNF) is a protein critically involved in neuronal growth and metabolism. BDNF also has been shown to be important regulator of oocyte maturation. Recent data show that BDNF can be potentially involved in POI pathology. The aim of the study was to assess the BDNF plasma concentrations in patients diagnosed with idiopathic POI. 23 women diagnosed with POI (age 31 ± 7 years) and 18 (age 31 ± 3) controls were included to the study, matched according to age and body mass index. The BDNF concentrations were measured using competitive enzyme-linked immunosorbent assay (ELISA). Hormonal and metabolic parameters were measured in all individuals, in controls in late follicular phase. The POI group demonstrated lower mean plasma concentrations of BDNF (429.25 ± 65.52 pg/ml) in comparison to healthy controls (479.75 ± 34.75 pg/ml, p = 0.0345). The BDNF plasma concentration correlated negatively (R = -0.79, p < 0.001) with number of months since last menstrual period. There was a positive correlation between BDNF and progesterone in controls. In conclusion, POI patients show significantly lower BDNF plasma concentration and it correlates with the duration of amenorrhea. This observation brings important potential insights to the pathology of POI.
卵巢早衰（POI）被定义为40岁以下女性卵巢功能的停止。脑源性神经营养因子（BDNF）是一种在神经元生长和代谢中起关键作用的蛋白质。BDNF也被证明是卵母细胞成熟的重要调节因子。最近的数据表明，BDNF可能参与POI的病理过程。本研究的目的是评估诊断为特发性POI患者的BDNF血浆浓度。23名诊断为POI的女性（年龄31±7岁）和18名（年龄31±3岁）对照者被纳入研究，根据年龄和体重指数进行匹配。使用竞争性酶联免疫吸附测定（ELISA）测量BDNF浓度。在所有个体中测量激素和代谢参数，对照者在卵泡晚期测量。与健康对照者（479.75±34.75 pg/ml，p = 0.0345）相比，POI组显示BDNF的平均血浆浓度较低（429.25±65.52 pg/ml）。BDNF血浆浓度与自上次月经以来的月数呈负相关（R = -0.79，p < 0.001）。在对照者中，BDNF与孕酮之间存在正相关。总之，POI患者的BDNF血浆浓度显著降低，且与闭经持续时间相关。这一观察结果为POI的病理研究带来了重要的潜在见解。
