Hodson S, Hodson G
Laboratory of Experimental Optometry, University of Wales Institute of Science and Technology, Cardiff, U.K.
Biochim Biophys Acta. 1988 Jan 22;937(2):241-6. doi: 10.1016/0005-2736(88)90246-5.
Membranes of ox corneal endothelial cells were studied for their surface area by electron microscopical stereology and then separated from homogenates on self forming Percoll gradients. Enzymic analysis of the plasma membrane-enriched fraction failed to demonstrate an ATPase activity which was stimulated by the presence of bicarbonate ions. It is proposed that the well-established bicarbonate secretion of these cells may be coupled to the plasma membrane (Na+ + K+)-ATPase activity by the stoichiometry of Na+/HCO3- close to 1:1.
通过电子显微镜体视学研究了牛角膜内皮细胞的细胞膜表面积，然后在自形成的Percoll梯度上从匀浆中分离出来。对富含质膜的部分进行酶分析，未能证明存在受碳酸氢根离子刺激的ATP酶活性。有人提出，这些细胞中已确定的碳酸氢根分泌可能通过接近1:1的Na⁺/HCO₃⁻化学计量与质膜（Na⁺ + K⁺）-ATP酶活性偶联。
