Pulido Pablo, Llamas Ernesto, Rodriguez-Concepcion Manuel
a Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB , Campus UAB Bellaterra , Barcelona , Spain.
Plant Signal Behav. 2017 Mar 4;12(3):e1290039. doi: 10.1080/15592324.2017.1290039.
Environmental stress conditions such as high light, extreme temperatures, salinity or drought trigger oxidative stress and eventually protein misfolding in plants. In chloroplasts, chaperone systems refold proteins after stress, while proteases degrade misfolded and aggregated proteins that cannot be refolded. We observed that reduced activity of chloroplast Hsp70 chaperone or Clp protease systems both prevented growth of Arabidopsis thaliana seedlings after treatment with the oxidative agent methyl viologen. Besides showing a role for these particular protein quality control components on the protection against oxidative stress, we provide evidence supporting the existence of a yet undiscovered pathway for Clp-mediated degradation of the damaged proteins.
高光、极端温度、盐度或干旱等环境胁迫条件会引发植物的氧化应激，并最终导致蛋白质错误折叠。在叶绿体中，伴侣系统在胁迫后重新折叠蛋白质，而蛋白酶则降解无法重新折叠的错误折叠和聚集的蛋白质。我们观察到，叶绿体Hsp70伴侣或Clp蛋白酶系统活性降低，均会阻止拟南芥幼苗在用氧化试剂甲基紫精处理后的生长。除了表明这些特定的蛋白质质量控制成分在抵御氧化应激方面的作用外，我们还提供了证据支持存在一条尚未被发现的Clp介导的受损蛋白质降解途径。
