单纯疱疹病毒复制过程中一种热休克70样蛋白的核内积累。

Nuclear accumulation of a heat-shock 70-like protein during herpes simplex virus replication.

作者信息

LaThangue N B, Latchman D S

机构信息

Imperial Cancer Research Fund, Tumour Immunology Unit, London.

出版信息

Biosci Rep. 1987 Jun;7(6):475-83. doi: 10.1007/BF01116504.

DOI:10.1007/BF01116504

PMID:2827804

Abstract

A monoclonal antibody defines an antigen, p68, related to hsp70, which is located in nuclei of uninfected exponential cells. Nuclear p68 is released by DNase but not RNase treatment suggesting an association with DNA. Lytic productive infection of confluent quiescent BHK 21 cells with herpes simplex virus type-2 causes p68 to accumulate in nuclei. The effect is specific for HSV-2, and does not occur in HSV-1 infected cells. Maximum nuclear accumulation of p68 requires virus DNA synthesis although a significant accumulation occurs in the absence of such synthesis. It is suggested that the nuclear accumulation of p68 is an aspect of a cellular stress response to lytic infection with HSV-2.

摘要

一种单克隆抗体可识别一种与热休克蛋白70（hsp70）相关的抗原p68，该抗原位于未感染的指数生长期细胞的细胞核中。核p68可被DNA酶释放，但RNA酶处理则不能，这表明它与DNA有关联。用2型单纯疱疹病毒对汇合静止的BHK 21细胞进行溶细胞性生产性感染，会导致p68在细胞核中积累。这种效应是2型单纯疱疹病毒特有的，在1型单纯疱疹病毒感染的细胞中不会出现。p68在细胞核中的最大积累需要病毒DNA合成，不过在没有这种合成的情况下也会有显著积累。有人提出，p68在细胞核中的积累是细胞对2型单纯疱疹病毒溶细胞性感染的应激反应的一个方面。