Zeng Sen, Ye Jianhua, Cui Zhixiang, Si Junhui, Wang Qianting, Wang Xiaofeng, Peng Kaiping, Chen Wenzhe
School of Materials Science and Engineering, Fuzhou University, Fujian 350000, China.
School of Materials Science and Engineering, Fuzhou University, Fujian 350000, China; School of Materials Science and Engineering, Fujian University of Technology, Fujian 350118, China; Fujian Provincial Key Laboratory of Advanced Materials Processing and Application, Fujian 350118, China; National Center for International Research of Micro-nano Molding Technology & Key Laboratory for Micro Molding Technology of Henan Province, Zhengzhou University, Henan 450001, China.
Mater Sci Eng C Mater Biol Appl. 2017 Aug 1;77:92-101. doi: 10.1016/j.msec.2017.03.220. Epub 2017 Mar 27.
As one of the stimulators on bone formation, osteogenic growth peptide (OGP) improves both proliferation and differentiation of the bone cells in vitro and in vivo. The aim of this work was the preparation of three dimensional porous poly(lactic acid) (PLA) scaffold with high porosity from PLA-dioxane-water ternary system with the use of vacuum-assisted solvent casting, phase separation, solvent extraction and particle leaching methods. Then, by surface coating of PLA scaffold with chitosan (CS)/OGP solution, biofunctionalization of PLA scaffold had been completed for application in bone regeneration. The effects of frozen temperature (-20, -50, -80°C) and PLA solution concentration (10, 12, 14wt%) on the microstructure, water absorption, porosity, hydrophilicity, mechanical properties, and biocompatibility of PLA and CS/OGP/PLA scaffold were investigated. Results showed that both PLA and CS/OGP/PLA scaffolds have an interconnected network structure and a porosity of up to 96.1% and 91.5%, respectively. The CS/OGP/PLA scaffold exhibited better hydrophilicity and mechanical properties than that of uncoated PLA scaffold. Moreover, the results of cell culture test showed that CS/OGP coating could stimulate the proliferation and growth of osteoblast cells on CS/OGP/PLA scaffold. These finding suggested that the surface biofunctionalization by CS/OGP coating layer could be an effective method on enhancing cell adhesion to synthetic polymer-based scaffolds in tissue engineering application and the developed porous CS/OGP/PLA scaffold should be considered as alternative biomaterials for bone regeneration.
作为骨形成的刺激因子之一，成骨生长肽（OGP）在体外和体内均可促进骨细胞的增殖与分化。本研究旨在利用真空辅助溶剂浇铸、相分离、溶剂萃取和粒子沥滤法，从聚乳酸-二氧六环-水三元体系制备具有高孔隙率的三维多孔聚乳酸（PLA）支架。然后，通过用壳聚糖（CS）/OGP溶液对PLA支架进行表面涂层，完成了PLA支架的生物功能化，以应用于骨再生。研究了冷冻温度（-20、-50、-80°C）和PLA溶液浓度（10、12、14wt%）对PLA以及CS/OGP/PLA支架的微观结构、吸水性、孔隙率、亲水性、力学性能和生物相容性的影响。结果表明，PLA和CS/OGP/PLA支架均具有相互连通的网络结构，孔隙率分别高达96.1%和91.5%。CS/OGP/PLA支架比未涂层的PLA支架表现出更好的亲水性和力学性能。此外，细胞培养测试结果表明，CS/OGP涂层可刺激成骨细胞在CS/OGP/PLA支架上的增殖和生长。这些发现表明，CS/OGP涂层进行的表面生物功能化可能是增强组织工程应用中细胞对合成聚合物基支架粘附的有效方法，所制备的多孔CS/OGP/PLA支架应被视为骨再生的替代生物材料。
