Jacques-Jamin Carine, Jeanjean-Miquel Corinne, Domergue Anaïs, Bessou-Touya Sandrine, Duplan Hélène
Pharmacologie Division 2 et Pharmacocinétique Cutanée, Centre R&D Pierre Fabre, Pierre Fabre Dermo-Cosmétique, Toulouse, France.
Skin Pharmacol Physiol. 2017;30(2):55-65. doi: 10.1159/000455196. Epub 2017 Mar 10.
Information is lacking on the dermal penetration of topically applied formulations on in vitro skin models, under conditions where the stratum corneum (SC) is damaged. Therefore, we have developed a standardized in vitro barrier-disrupted skin model using tape stripping.
Different tape stripping conditions were evaluated using histology, transepidermal water loss, infrared densitometry, and caffeine absorption.
The effects of tape stripping were comparable using pig and human skin. Optimized conditions were used to test the effect of SC damage and UV irradiation on the absorption of an UV filter combination present in a sunscreen. The bioavailability of the filters was extremely low regardless of the extent of skin damage, suggesting bioavailability would not be increased if the consumer applied the sunscreen to sun-damaged skin.
This standardized in vitro methodology using pig or human skin for damaged skin will add valuable information for the safety assessment of topically applied products.
在角质层（SC）受损的情况下，关于局部应用制剂在体外皮肤模型上的皮肤渗透信息尚缺。因此，我们开发了一种使用胶带剥离的标准化体外屏障破坏皮肤模型。
使用组织学、经表皮水分流失、红外密度测定法和咖啡因吸收来评估不同的胶带剥离条件。
使用猪皮和人皮时，胶带剥离的效果相当。优化后的条件用于测试SC损伤和紫外线照射对防晒剂中紫外线过滤剂组合吸收的影响。无论皮肤损伤程度如何，过滤剂的生物利用度都极低，这表明如果消费者将防晒剂涂抹在晒伤的皮肤上，生物利用度不会提高。
这种使用猪皮或人皮的标准化体外受损皮肤方法将为局部应用产品的安全性评估增添有价值的信息。
