Wrobeln Anna, Schlüter Klaus D, Linders Jürgen, Zähres Manfred, Mayer Christian, Kirsch Michael, Ferenz Katja B
a Institute for Physiological Chemistry, University Hospital Essen, University of Duisburg-Essen , Essen , Germany.
b Institute of Physiology, Justus-Liebig-University , Giessen , Germany.
Artif Cells Nanomed Biotechnol. 2017 Jun;45(4):723-730. doi: 10.1080/21691401.2017.1284858. Epub 2017 Feb 8.
The aim of this study was to prove whether albumin-derived perfluorocarbon-based nanoparticles (capsules) can operate as a novel artificial oxygen carrier in a rat Langendorff-heart perfusion model. Hearts perfused with capsules showed increased left ventricular pressure and rate pressure product compared to hearts perfused with pure Krebs-Henseleit (KH)-buffer. The capsules prevented the myocardium from functional fail when in their absence a noxious ischemia was observed. Capsules did not change rheological properties of KH-buffer and could repeatedly reload with oxygen. This albumin-derived perfluorocarbon-based artificial oxygen carrier preserved the function of rat hearts due to the transport of oxygen in a satisfactory manner. Because of these positive results, the functionality of the applied capsules should be verified in living animals.
本研究的目的是证明基于白蛋白的全氟化碳纳米颗粒（胶囊）是否能在大鼠Langendorff心脏灌注模型中作为一种新型人工氧载体发挥作用。与用纯Krebs-Henseleit（KH）缓冲液灌注的心脏相比，用胶囊灌注的心脏左心室压力和心率压力乘积增加。当不存在胶囊时观察到有害的缺血，而胶囊可防止心肌功能衰竭。胶囊不会改变KH缓冲液的流变学性质，并且可以反复充氧。这种基于白蛋白的全氟化碳人工氧载体以令人满意的方式输送氧气，从而保留了大鼠心脏的功能。鉴于这些积极结果，应在活体动物中验证所用胶囊的功能。
