Carroll John F, Demirci Betul, Kramer Matthew, Bernier Ulrich R, Agramonte Natasha M, Baser K Husnu Can, Tabanca Nurhayat
a USDA, ARS, Invasive Insects Biocontrol and Behavior Laboratory , Beltsville Agricultural Research Center , Beltsville , MD , (retired) USA.
b Faculty of Pharmacy, Department of Pharmacognosy , Anadolu University , Eskisehir , Turkey.
Nat Prod Res. 2017 Sep;31(18):2192-2197. doi: 10.1080/14786419.2017.1280485. Epub 2017 Feb 16.
The oregano, Origanum onites L., essential oil (EO) was tested in laboratory behavioural bioassays for repellent activity against Amblyomma americanum (L.) and Aedes aegypti (L.). The O. onites EO was characterised using GC-FID and GC-MS. Carvacrol (75.70%), linalool (9.0%), p-cymene (4.33%) and thymol (1.9%) were the most abundant compounds. At a concentration of 0.413 mg oil/cm of filter paper, O. onites EO repelled 100% of the ticks tested and at 0.103 mg oil/cm of filter paper, 66.7% of the ticks were repelled. At 0.075 mg oil/cm filter paper, thymol repelled 66.7% of the ticks compared to 28.7% by carvacrol at that same concentration. Against Ae. aegypti, O. onites EO was repellent at the minimum effective dosage (MED) of 0.011 (±0.00) mg/cm in the cloth patch assay compared to the reference control, N,N-dimethyl-3-methylbenzamide (DEET) with a MED = 0.007 ± (0.003) mg/cm.
牛至（Origanum onites L.）精油（EO）在实验室行为生物测定中测试了对美洲钝眼蜱（Amblyomma americanum (L.)）和埃及伊蚊（Aedes aegypti (L.)）的驱避活性。使用气相色谱 - 火焰离子化检测器（GC - FID）和气相色谱 - 质谱联用仪（GC - MS）对牛至EO进行了表征。香芹酚（75.70%）、芳樟醇（9.0%）、对伞花烃（4.33%）和百里酚（1.9%）是含量最高的化合物。在滤纸浓度为0.413毫克油/平方厘米时，牛至EO驱避了100%的受试蜱虫；在滤纸浓度为0.103毫克油/平方厘米时，66.7%的蜱虫被驱避。在滤纸浓度为0.075毫克油/平方厘米时，百里酚驱避了66.7%的蜱虫，而在相同浓度下香芹酚驱避率为28.7%。对于埃及伊蚊，在布片试验中，与参考对照N,N - 二甲基 - 3 - 甲基苯甲酰胺（避蚊胺，DEET）相比，牛至EO在最低有效剂量（MED）为0.011（±0.00）毫克/平方厘米时具有驱避作用，避蚊胺的MED = 0.007 ±（0.003）毫克/平方厘米。
