Moazzami Fariborz, Asheghi Bahar, Sahebi Safoura
Dept. Endodontic, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
J Dent (Shiraz). 2017 Mar;18(1):24-29.
The maintenance of viable periodontal ligament cells is the most important issue in the long-term preservation of avulsed teeth.
The aim of this study was to assess aloe vera as a new storage media in maintaining the cell viability of dry-stored teeth in comparison with soy milk, Hank`s balanced salt solution (HBSS), and milk.
Twenty one extracted dog premolar teeth were dried for 30 minutes and stored in soy milk, HBSS, milk, and aloe vera extract (50%) for 45 minutes (n=6 for each). Furthermore, positive and two negative control groups (n=6), corresponding to 0 min, 30 min, and 2-hour drying times were also prepared respectively. The number of viable cells was counted following storage using Trypan blue exclusion. Data were statistically analyzed using the one-way ANOVA and post hoc Tukey-HSD test.
Statistical analysis showed no significant differences in cell viability among aloe vera, soymilk, and HBSS- stored teeth; however, they were all superior to milk.
Aloe vera extract can be recommended as a suitable storage media for avulsed teeth.
维持牙周膜细胞的活力是离体牙长期保存中最重要的问题。
本研究旨在评估芦荟作为一种新的储存介质，与豆浆、汉克斯平衡盐溶液（HBSS）和牛奶相比，在维持干储存牙齿的细胞活力方面的效果。
21颗拔除的犬前磨牙干燥30分钟，然后分别储存在豆浆、HBSS、牛奶和芦荟提取物（50%）中45分钟（每组n = 6）。此外，还分别制备了对应0分钟、30分钟和2小时干燥时间的阳性和两个阴性对照组（每组n = 6）。储存后使用台盼蓝排斥法计数活细胞数量。数据采用单因素方差分析和事后Tukey-HSD检验进行统计学分析。
统计分析表明，芦荟、豆浆和HBSS储存的牙齿之间细胞活力无显著差异；然而，它们均优于牛奶。
芦荟提取物可推荐作为离体牙合适的储存介质。
