Navit Saumya, Shahi Niharika, Khan Suleman Abbas, Sharma Anshul, Singh Vartika, Mishra Ratna Priya, Navit Pragati, Sharma Prerna
Professor and Head of Department, Department of Pedodontics and Preventive Dentistry, Saraswati Dental College, Lucknow, Uttar Pradesh, India.
Postgraduate Student, Department of Pedodontics and Preventive Dentistry, Saraswati Dental College, Lucknow, Uttar Pradesh, India.
J Clin Diagn Res. 2017 Jun;11(6):ZC01-ZC04. doi: 10.7860/JCDR/2017/26673.9954. Epub 2017 Jun 1.
Dentoalveolar trauma is a very common and avulsion is the most frequent injury. Treatment of choice following avulsion is immediate reimplantation. However, in cases where this might not be possible the prognosis of reimplanted teeth can be improved by the selection of an appropriate storage media. Prevention of ankylosis and replacement resorption depends more on the potential of storage media to maintain cell viability rather than the extraalveolar time period.
To compare and evaluate the efficacy of different storage media in maintaining the viability of Periodontal Ligament (PDL) cells in an avulsed tooth.
A total of 58 freshly extracted human premolar teeth were divided into four experimental groups and two control groups. The positive and negative controls corresponded to 0 minute and an eight hours dry time, respectively. The experimental teeth were stored dry for 30 minutes and then immersed in one of the four media: Hank's Balanced Salt Solution (HBSS), coconut water, aloe vera and saline for 45 minutes. The teeth were then treated with Collagenase Type II and Dispase for 30 minutes. The number of viable PDL cells were counted with a haemocytometer and analysed. The statistical analysis was done using SPSS version 15.0. The Analysis of Variance (ANOVA) and Post-hoc tests (Tukey-HSD) were performed to reveal the statistical significance. The values were represented in number (%) and mean±SD.
Statistical analysis demonstrated that among the experimental groups maximum percentage of viable cells were seen in HBSS (87.33%) followed by coconut water (79.87%), aloe vera (70.59%) and saline (50.56%).
Within the parameters of this study, it can be concluded that the HBSS is the most effective storage media in maintaining the viability of PDL cells. However, in Indian scenario where availability of HBSS is questionable, coconut water can be considered as the best natural storage media for an avulsed tooth followed by aloe vera.
牙槽突创伤非常常见，其中牙脱位是最常见的损伤类型。牙脱位后的首选治疗方法是立即再植。然而，在无法立即再植的情况下，选择合适的储存介质可改善再植牙的预后。防止牙根粘连和替代性吸收更多地取决于储存介质维持细胞活力的能力，而非脱出牙槽窝后的时间。
比较和评估不同储存介质对脱位牙牙周膜（PDL）细胞活力的维持效果。
将58颗新鲜拔除的人类前磨牙分为四个实验组和两个对照组。阳性和阴性对照分别对应0分钟和8小时干燥时间。实验牙干燥保存30分钟，然后浸入四种介质之一：汉克斯平衡盐溶液（HBSS）、椰子水、芦荟和生理盐水，浸泡45分钟。然后用II型胶原酶和 dispase处理牙齿30分钟。用血细胞计数器对存活的PDL细胞数量进行计数并分析。使用SPSS 15.0版本进行统计分析。采用方差分析（ANOVA）和事后检验（Tukey-HSD）来揭示统计学意义。数值以数量（%）和平均值±标准差表示。
统计分析表明，在实验组中，HBSS组存活细胞百分比最高（87.33%），其次是椰子水（79.87%）、芦荟（70.59%）和生理盐水（50.56%）。
在本研究的参数范围内，可以得出结论，HBSS是维持PDL细胞活力最有效的储存介质。然而，在印度，HBSS的可用性存在疑问，椰子水可被视为脱位牙的最佳天然储存介质，其次是芦荟。
